Laterally Confined Microfluidic Patterning of Cells for Engineering Spatially Defined Vascularization

Hojatollah Rezaei Nejad; Zahra Goli Malekabadi; Mehdi Kazemzadeh Narbat; Nasim Annabi; Pooria Mostafalu; Farhang Tarlan; Yu Shrike Zhang; Mina Hoorfar; Ali Tamayol; Ali Khademhosseini

doi:10.1002/smll.201601391

Laterally Confined Microfluidic Patterning of Cells for Engineering Spatially Defined Vascularization

Small. 2016 Oct;12(37):5132-5139. doi: 10.1002/smll.201601391. Epub 2016 Aug 11.

Authors

Hojatollah Rezaei Nejad^{1

2

3}, Zahra Goli Malekabadi^{1

2

4}, Mehdi Kazemzadeh Narbat^{1

2}, Nasim Annabi^{1

2

5

6}, Pooria Mostafalu^{1

2

6}, Farhang Tarlan^{1

2}, Yu Shrike Zhang^{1

2

6}, Mina Hoorfar³, Ali Tamayol^{7

8

9}, Ali Khademhosseini^{10

11

12

13

14}

Affiliations

¹ Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA.
² Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
³ Department of Mechanical Engineering, University of British Columbia, Kelowna, BC, Canada, V1V 1V7.
⁴ Department of Mechanical Engineering, Amirkabir University of Technology, Tehran, Iran.
⁵ Department of Chemical Engineering, Northeastern University, Boston, MA, 02115-5000, USA.
⁶ Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
⁷ Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA. [email protected].
⁸ Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. [email protected].
⁹ Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA. [email protected].
¹⁰ Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02139, USA. [email protected].
¹¹ Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. [email protected].
¹² Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA. [email protected].
¹³ Department of Physics, King Abdulaziz University, Jeddah, 21569, Saudi Arabia. [email protected].
¹⁴ Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul, 05029, Republic of Korea. [email protected].

Abstract

A biofabrication strategy for creating planar multiscale protein, hydrogel, and cellular patterns, and simultaneously generating microscale topographical features is developed that laterally confines the patterned cells and direct their growth in cell permissive hydrogels.

Keywords: capillary-driven microfluidics; cell patterning; hydrogels; microfabrication; vascularization.

MeSH terms

Actins / metabolism
Animals
Human Umbilical Vein Endothelial Cells / drug effects
Human Umbilical Vein Endothelial Cells / metabolism*
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology
Microfluidics / methods*
Neovascularization, Physiologic* / drug effects
Staining and Labeling
Sus scrofa
Tissue Engineering / methods*

Substances

Actins
Hydrogel, Polyethylene Glycol Dimethacrylate

Abstract

MeSH terms

Substances

Grants and funding