Purification and characterization of mouse hematopoietic stem cells

Science. 1988 Jul 1;241(4861):58-62. doi: 10.1126/science.2898810.

Abstract

Mouse bone marrow hematopoietic stem cells were isolated with the use of a variety of phenotypic markers. These cells can proliferate and differentiate with approximately unit efficiency into myelomonocytic cells, B cells, or T cells. Thirty of these cells are sufficient to save 50 percent of lethally irradiated mice, and to reconstitute all blood cell types in the survivors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Surface / immunology
  • B-Lymphocytes / cytology
  • Bone Marrow Cells*
  • Cell Count
  • Cell Differentiation
  • Cell Division
  • Cell Separation / methods*
  • Colony-Forming Units Assay
  • Erythrocytes / cytology
  • Granulocytes / cytology
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / immunology
  • Mice
  • Mice, Inbred C57BL
  • Spleen / cytology
  • T-Lymphocytes / cytology
  • Thy-1 Antigens
  • Thymus Gland / cytology

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Thy-1 Antigens