A comprehensive analysis of polymorphic variants in steroid hormone and insulin-like growth factor-1 metabolism and risk of in situ breast cancer: Results from the Breast and Prostate Cancer Cohort Consortium

Int J Cancer. 2018 Mar 15;142(6):1182-1188. doi: 10.1002/ijc.31145. Epub 2017 Nov 17.

Abstract

We assessed the association between 1,414 single nucleotide polymorphisms (SNPs) in genes involved in synthesis and metabolism of steroid hormones and insulin-like growth factor 1, and risk of breast cancer in situ (BCIS), with the aim of determining whether any of these were disease specific. This was carried out using 1,062 BCIS cases and 10,126 controls as well as 6,113 invasive breast cancer cases from the Breast and Prostate Cancer Cohort Consortium (BPC3). Three SNPs showed at least one nominally significant association in homozygous minor versus homozygous major models. ACVR2A-rs2382112 (ORhom = 3.05, 95%CI = 1.72-5.44, Phom = 1.47 × 10-4 ), MAST2-rs12124649 (ORhom = 1.73, 95% CI =1.18-2.54, Phom = 5.24 × 10-3 ), and INSR-rs10500204 (ORhom = 1.96, 95% CI = 1.44-2.67, Phom =1.68 × 10-5 ) were associated with increased risk of BCIS; however, only the latter association was significant after correcting for multiple testing. Furthermore, INSR-rs10500204 was more strongly associated with the risk of BCIS than invasive disease in case-only analyses using the homozygous minor versus homozygous major model (ORhom = 1.78, 95% CI = 1.30-2.44, Phom = 3.23 × 10-4 ). The SNP INSR-rs10500204 is located in an intron of the INSR gene and is likely to affect binding of the promyelocytic leukemia (PML) protein. The PML gene is known as a tumor suppressor and growth regulator in cancer. However, it is not clear on what pathway the A-allele of rs10500204 could operate to influence the binding of the protein. Hence, functional studies are warranted to investigate this further.

Keywords: BPC3; breast cancer in situ; genetic epidemiology; single nucleotide polymorphisms.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activin Receptors, Type II / genetics*
  • Activin Receptors, Type II / metabolism
  • Aged
  • Antigens, CD / genetics*
  • Antigens, CD / metabolism
  • Breast Carcinoma In Situ / genetics*
  • Breast Neoplasms / genetics*
  • Case-Control Studies
  • Female
  • Genetic Predisposition to Disease / genetics
  • Gonadal Steroid Hormones / metabolism
  • Humans
  • Insulin-Like Growth Factor I / metabolism
  • Introns / genetics
  • Microtubule-Associated Proteins / genetics*
  • Microtubule-Associated Proteins / metabolism
  • Middle Aged
  • Polymorphism, Single Nucleotide / genetics
  • Promyelocytic Leukemia Protein / metabolism
  • Prospective Studies
  • Protein Serine-Threonine Kinases / genetics*
  • Protein Serine-Threonine Kinases / metabolism
  • Receptor, Insulin / genetics*
  • Receptor, Insulin / metabolism

Substances

  • Antigens, CD
  • Gonadal Steroid Hormones
  • IGF1 protein, human
  • Microtubule-Associated Proteins
  • Promyelocytic Leukemia Protein
  • PML protein, human
  • Insulin-Like Growth Factor I
  • INSR protein, human
  • Receptor, Insulin
  • MAST2 protein, human
  • Protein Serine-Threonine Kinases
  • Activin Receptors, Type II
  • activin receptor type II-A