Randomised trial of epinephrine dose and flush volume in term newborn lambs

Arch Dis Child Fetal Neonatal Ed. 2021 Nov;106(6):578-583. doi: 10.1136/archdischild-2020-321034. Epub 2021 Mar 9.

Abstract

Objectives: Neonatal resuscitation guidelines recommend 0.5-1 mL saline flush following 0.01-0.03 mg/kg of epinephrine via low umbilical venous catheter for persistent bradycardia despite effective positive pressure ventilation (PPV) and chest compressions (CC). We evaluated the effects of 1 mL vs 3 mL/kg flush volumes and 0.01 vs 0.03 mg/kg doses on return of spontaneous circulation (ROSC) and epinephrine pharmacokinetics in lambs with cardiac arrest.

Design: Forty term lambs in cardiac arrest were randomised to receive 0.01 or 0.03 mg/kg epinephrine followed by 1 mL or 3 mL/kg flush after effective PPV and CC. Epinephrine (with 1 mL flush) was repeated every 3 min until ROSC or until 20 min. Haemodynamics, blood gases and plasma epinephrine concentrations were monitored.

Results: Ten lambs had ROSC before epinephrine administration and 2 died during instrumentation. Among 28 lambs that received epinephrine, 2/6 in 0.01 mg/kg-1 mL flush, 3/6 in 0.01 mg/kg-3 mL/kg flush, 5/7 in 0.03 mg/kg-1 mL flush and 9/9 in 0.03 mg/kg-3 mL/kg flush achieved ROSC (p=0.02). ROSC was five times faster with 0.03 mg/kg epinephrine compared with 0.01 mg/kg (adjusted HR (95% CI) 5.08 (1.7 to 15.25)) and three times faster with 3 mL/kg flush compared with 1 mL flush (3.5 (1.27 to 9.71)). Plasma epinephrine concentrations were higher with 0.01 mg/kg-3 mL/kg flush (adjusted geometric mean ratio 6.0 (1.4 to 25.7)), 0.03 mg/kg-1 mL flush (11.3 (2.1 to 60.3)) and 0.03 mg/kg-3 mL/kg flush (11.0 (2.2 to 55.3)) compared with 0.01 mg/kg-1 mL flush.

Conclusions: 0.03 mg/kg epinephrine dose with 3 mL/kg flush volume is associated with the highest ROSC rate, increases peak plasma epinephrine concentrations and hastens time to ROSC. Clinical trials evaluating optimal epinephrine dose and flush volume are warranted.

Keywords: cardiology; neonatology; resuscitation.

MeSH terms

  • Adrenergic beta-Agonists / administration & dosage
  • Adrenergic beta-Agonists / pharmacokinetics
  • Animals
  • Animals, Newborn
  • Bradycardia* / blood
  • Bradycardia* / drug therapy
  • Bradycardia* / etiology
  • Cardiopulmonary Resuscitation / methods*
  • Catheterization, Peripheral / methods
  • Coronary Circulation / drug effects*
  • Dose-Response Relationship, Drug
  • Drug Monitoring / methods
  • Epinephrine* / administration & dosage
  • Epinephrine* / blood
  • Epinephrine* / pharmacokinetics
  • Heart Arrest* / physiopathology
  • Heart Arrest* / therapy
  • Heart Massage / methods
  • Positive-Pressure Respiration / methods
  • Sheep
  • Treatment Outcome
  • Umbilical Veins

Substances

  • Adrenergic beta-Agonists
  • Epinephrine