Molecular cloning and expression of a novel peptide (LN1) gene: reduced expression in the renal cortex of lupus nephritis in MRL/lpr mouse

Biochem Biophys Res Commun. 1996 Dec 4;229(1):355-60. doi: 10.1006/bbrc.1996.1805.

Abstract

A gene has been identified by mRNA differential display whose expression is reduced in the renal cortex of MRL/lpr mouse. The nucleotide sequence of the cDNA contains an open reading frame that encodes a protein of 338 amino acids (termed LN1). In situ hybridization showed that LN1 mRNA is present in glomeruli, and a 39 kDa protein was detected in the kidney by immunoblot. A human LN1 cDNA was also isolated, the deduced amino acid sequence of which is 78% identical to that of mouse LN1. Although the function of LN1 remains to be elucidated, its reduced expression may contribute to the pathogenesis of lupus nephritis.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / isolation & purification*
  • Humans
  • In Situ Hybridization
  • Kidney Cortex / chemistry*
  • Kidney Glomerulus / chemistry*
  • Lupus Nephritis / etiology
  • Lupus Nephritis / genetics*
  • Lupus Nephritis / pathology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred MRL lpr
  • Molecular Sequence Data
  • Plant Proteins*
  • RNA, Messenger / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Time Factors
  • Tissue Distribution

Substances

  • Homeodomain Proteins
  • Kn1 protein, plant
  • Plant Proteins
  • RNA, Messenger

Associated data

  • GENBANK/D88670