Metabolic profiling reveals local and systemic responses of host plants to nematode parasitism

Plant J. 2010 Jun 1;62(6):1058-71. doi: 10.1111/j.1365-313X.2010.04217.x. Epub 2010 Mar 31.

Abstract

The plant parasitic beet cyst nematode Heterodera schachtii induces syncytial feeding structures in Arabidopsis roots. The feeding structures form strong sink tissues that have been suggested to be metabolically highly active. In the present study, metabolic profiling and gene targeted expression analyses were performed in order to study the local and systemic effects of nematode infection on the plant host. The results showed increased levels of many amino acids and phosphorylated metabolites in syncytia, as well as high accumulation of specific sugars such as 1-kestose that do not accumulate naturally in Arabidopsis roots. A correlation-based network analysis revealed highly activated and coordinated metabolism in syncytia compared to non-infected control roots. An integrated analysis of the central primary metabolism showed a clear coherence of metabolite and transcript levels, indicating transcriptional regulation of specific pathways. Furthermore, systemic effects of nematode infection were demonstrated by correlation-based network analysis as well as independent component analysis. 1-kestose, raffinose, alpha,alpha-trehalose and three non-identified analytes showed clear systemic accumulation, indicating future potential for diagnostic and detailed metabolic analyses. Our studies open the door towards understanding the complex remodelling of plant metabolism in favour of the parasitizing nematode.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis / parasitology
  • Gas Chromatography-Mass Spectrometry
  • Gene Expression Regulation, Plant
  • Giant Cells / metabolism
  • Metabolome*
  • Nematoda / physiology*
  • Plant Diseases / genetics*
  • Plant Diseases / parasitology
  • Plant Roots / metabolism
  • RNA, Plant / genetics
  • Raffinose / metabolism
  • Trehalose / metabolism
  • Trisaccharides / metabolism

Substances

  • RNA, Plant
  • Trisaccharides
  • 1-kestose
  • Trehalose
  • Raffinose