Anti-Pseudomonas aeruginosa IgY antibodies promote bacterial opsonization and augment the phagocytic activity of polymorphonuclear neutrophils

Hum Vaccin Immunother. 2016 Jul 2;12(7):1690-9. doi: 10.1080/21645515.2016.1145848. Epub 2016 Feb 22.

Abstract

Moderation of polymorphonuclear neutrophils (PMNs) as part of a critical defense against invading pathogens may offer a promising therapeutic approach to supplement the antibiotic eradication of Pseudomonas aeruginosa infection in non-chronically infected cystic fibrosis (CF) patients. We have observed that egg yolk antibodies (IgY) harvested from White leghorn chickens that target P. aeruginosa opsonize the pathogen and enhance the PMN-mediated respiratory burst and subsequent bacterial killing in vitro. The effects on PMN phagocytic activity were observed in different Pseudomonas aeruginosa strains, including clinical isolates from non-chronically infected CF patients. Thus, oral prophylaxis with anti-Pseudomonas aeruginosa IgY may boost the innate immunity against Pseudomonas aeruginosa in the CF setting by facilitating a rapid and prompt bacterial clearance by PMNs.

Keywords: IgY; cystic fibrosis; phagocytosis; pseudomonas aeruginosa.

MeSH terms

  • Animals
  • Antibodies, Bacterial / administration & dosage
  • Antibodies, Bacterial / immunology*
  • Chickens
  • Healthy Volunteers
  • Humans
  • Immunization, Passive / methods
  • Immunoglobulins / administration & dosage
  • Immunoglobulins / immunology*
  • Microbial Viability / drug effects
  • Neutrophils / immunology*
  • Opsonin Proteins / administration & dosage
  • Opsonin Proteins / immunology*
  • Phagocytosis*
  • Pseudomonas Infections / therapy
  • Pseudomonas aeruginosa / immunology*

Substances

  • Antibodies, Bacterial
  • IgY
  • Immunoglobulins
  • Opsonin Proteins