The use of fluorescently-tagged apoptolidins in cellular uptake and response studies

J Antibiot (Tokyo). 2016 Apr;69(4):327-30. doi: 10.1038/ja.2016.22. Epub 2016 Mar 9.

Abstract

The apoptolidins are glycomacrolide microbial metabolites reported to be selectively cytotoxic against tumor cells. Using fluorescently tagged active derivatives we demonstrate selective uptake of these four tagged glycomacrolides in cancer cells over healthy human blood cells. We also demonstrate the utility of these five fluorescently tagged glycomacrolides in fluorescent flow cytometry to monitor cellular uptake of the six glycomacrolides and cellular response.

MeSH terms

  • A549 Cells
  • Biological Transport
  • Cell Line, Tumor
  • Flow Cytometry
  • Fluorescent Dyes
  • Humans
  • Macrolides / chemistry
  • Macrolides / metabolism*
  • Staining and Labeling

Substances

  • Fluorescent Dyes
  • Macrolides
  • apoptolidin