Gingerenone A, a polyphenol present in ginger, suppresses obesity and adipose tissue inflammation in high-fat diet-fed mice

Mol Nutr Food Res. 2017 Oct;61(10):10.1002/mnfr.201700139. doi: 10.1002/mnfr.201700139. Epub 2017 Jul 20.

Abstract

Scope: Ginger exerts protective effects on obesity and its complications. Our objectives here are to identify bioactive compounds that inhibit adipogenesis and lipid accumulation in vitro, elucidate the anti-obesity effect of gingerenone A (GA) in diet-induced obesity (DIO), and investigate whether GA affects adipose tissue inflammation (ATI).

Methods and results: Oil red O staining showed that GA had the most potent inhibitory effect on adipogenesis and lipid accumulation in 3T3-L1 cells among ginger components tested at a single concentration (40 μM). Consistent with in vitro data, GA attenuates DIO by reducing fat mass in mice. This was accompanied by a modulation of fatty acid metabolism via activation of AMP-activated protein kinase (AMPK) in vitro and in vivo. Additionally, GA suppressed ATI by inhibiting macrophage recruitment and downregulating pro-inflammatory cytokines.

Conclusion: These results suggest that GA may be used as a potential therapeutic candidate for the treatment of obesity and its complications by suppressing adipose expansion and inflammation.

Keywords: AMP-activated protein kinase; Adipocyte; Adipose tissue inflammation; Gingerenone A; Obesity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • AMP-Activated Protein Kinases / genetics
  • AMP-Activated Protein Kinases / metabolism
  • Adipocytes / cytology
  • Adipocytes / drug effects
  • Adipogenesis / drug effects
  • Adiposity / drug effects
  • Animals
  • Anti-Obesity Agents / pharmacology*
  • Chemokine CCL2 / genetics
  • Chemokine CCL2 / metabolism
  • Cholesterol / blood
  • Coculture Techniques
  • Diarylheptanoids / pharmacology*
  • Diet, High-Fat
  • Fatty Acids, Nonesterified / blood
  • Gene Expression Regulation
  • Inflammation / drug therapy*
  • Lipid Metabolism / drug effects
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity / drug therapy*
  • Polyphenols / pharmacology*
  • RAW 264.7 Cells
  • Triglycerides / blood
  • Zingiber officinale / chemistry*

Substances

  • Anti-Obesity Agents
  • Ccl2 protein, mouse
  • Chemokine CCL2
  • Diarylheptanoids
  • Fatty Acids, Nonesterified
  • Polyphenols
  • Triglycerides
  • gingerenone A
  • Cholesterol
  • AMP-Activated Protein Kinases