A novel potential treatment technique applied to a glucose biosensor that is based on
pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) and chromium
hexacyanoferrate (CrHCF) incorporated into a platinum (Pt) electrode was demonstrated.
CrHCF, serving as a mediator, was electrochemically deposited on the Pt electrode as
ascertained by CV, SEM, FTIR and XPS measurements. The potential treatment of CrHCF,
which converts Fe (II) to Fe (III), enables the glucose detection. The amperometric …

A pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase based platinum
electrode was developed to detect glucose. Chromium hexacyanoferrate was modified onto
this electrode to serve as an electron transfer mediator between PQQ and the platinum
electrode. This biosensor showed the optimal response of glucose measurements at pH 7
and an operation potential of+ 0.4 V (vs. Ag/AgCl). More importantly the lifespan of the
biosensor has been last for 47 days without significant enzyme activity degradation.