Izvorni kod stranice Sterol 14-demetilaza

{{Infobox Enzim
| Name = Sterol 14-demetilaza
| EC_number = 1.14.13.70
| CAS_number = 60063-87-8
| IUBMB_EC_number = 1/14/13/70
| GO_code = 
| image = 
| width = 
| caption =
}}
'''Sterol 14-demetilaza''' ({{EC broj|1.14.13.70}}, ''obtusufoliolna 14-demetilaza'', ''lanosterolna 14-demetilaza'', ''lanosterolna 14alfa-demetilaza'', ''sterolna 14alfa-demetilaza'') je [[enzim]] sa sistematskim imenom ''sterol,NADPH:kiseonik oksidoreduktaza (14-metil odvajanje)''.<ref>{{cite journal | title = Cloning and expression in ''Escherichia coli'' of the obtusifoliol 14&alpha;-demethylase of ''Sorghum bicolor'' (L.) Moench, a cytochrome ''P''<small><sub>450</sub></small> orthologous to the sterol 14&alpha;-demethylases (CYP51) from fungi and mammals | url = https://archive.org/details/sim_plant-journal_1997-02_11_2/page/191 |author = Bak, S., Kahn, R.A., Olsen, C.E. and Halkier, B.A. |journal = Plant J. |date = 1997 |volume = 11 |pages = 191-201 |pmid = 9076987}}</ref><ref>{{cite journal | title = Different substrate specificities of lanosterol 14&alpha;-demethylase (''P''<small><sub>450</sub></small>-14DM) of ''Saccharomyces cerevisiae'' and rat liver of 24-methylene-24,25-dihydrolanosterol and 24,25-dihydrolanosterol |author = Aoyama, Y. and Yoshida, Y. |journal = Biochem. Biophys. Res. Commun. |date = 1991 |volume = 178 |pages = 1064-1071 |pmid = 1872829}}</ref><ref>{{cite journal | title = The 4&beta;-methyl group of substrate does not affect the activity of lanosterol 14&alpha;-demethylase (''P''<small><sub>450</sub></small>14DM) of yeast: differences between the substrate recognition by yeast and plant sterol 14&alpha;-demethylases |author = Aoyama, Y. and Yoshida, Y. |journal = Biochem. Biophys. Res. Commun. |date = 1992 |volume = 183 |pages = 1266-1272 |pmid = 1567403}}</ref><ref>{{cite journal | title = The removal of the 32-carbon atom as formic acid in cholesterol biosynthesis |author = Alexander, K., Akhtar, M., Boar, R.B., McGhie, J.F. and Barton, D.H.R. |journal = J. Chem. Soc. Chem. Commun. |date = 1972 |volume =  |pages = 383-385 |pmid = }}</ref> Ovaj enzim [[Kataliza|katalizuje]] sledeću [[hemijska reakcija|hemijsku reakciju]]

: [[obtusifoliol]] + 3 O<sub>2</sub> + 3 NADPH + 3 H<sup>+</sup> <math>\rightleftharpoons</math> 4alfa-metil-5alfa-ergosta-8,14,24(28)-trien-3beta-ol + format + 3 NADP<sup>+</sup> + 4H<sub>2</sub>O

Ovaj [[hem]]-[[tiol]]atni enzim (P-450) katalizuje sukcesivne hidroksilacije 14alfa-metil grupe i C-15, čemu sledi eliminacija formata i formiranje 14(15) dvostruke veze.

== Reference ==
{{reflist}}

== Literatura ==
{{refbegin|2}}
* {{PriceEnzymology3rd}}
* {{TooneAdvancesInEnzymology}}
* {{Branden ProteinStructure2nd}}
* {{SegelEnzymeKinetics}}
* {{CopelandEvaluationOfEnzymeInhibitors2nd}}
* {{MichalBiochemicalPathways}}
{{refend}}

== Spoljašnje veze ==
* {{MeshName|Sterol+14-demethylase}}

{{Enzimi}}

[[Kategorija:EC 1.14.13]]