F. Besse Stocks (FB-STOCK)

FB-STOCK

Name	VDRC Id	Library	Sub-Category	Genotype	Comment	Construct Id	Publication	Provider Scientist	Provider Organization	Gene Symbol	Synonyms	Sequence	Comment	Gene Name	Keywords	Price (net)
310601	310601	FB-STOCK	tagged construct	w*; me31B[KI.mTomato]	The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.	310601	Formicola et al., eLife (2021)	Florence Besse	Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)	me31B	C-terminal mTomato tag DDX6 Dhh1p/Me31b DmRH3 ME31B Mat31B Maternal expression at 31B Me31B Me31b P-body marker knock-in l(2)k06607 mTomato maternal expression at 31B me31B me31b	see publication	The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.	maternal expression at 31B	P-body marker knock-in mTomato C-terminal mTomato tag	Sign In to show prices
310602	310602	FB-STOCK	tagged construct	w*; ; rin[KI.mCherry]	The mCherry sequence was inserted via Crispr/Cas9 in the rin locus, at the N-terminus of the rin coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mCherry. Rin is the Drosophila ortholog of the G3BP1; it is Stress Granule marker positive.	310602	Pushpalatha et al., Nat com (2022)	Florence Besse	Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)	rin	N-terminal mCherry tag Next to squid Nts RIN Rasputin Rin Stress Granule marker cg9412 knock-in mCherry rasp rasputin rin	see publication (Formicola et al., 2021; doi: 10.7554/eLife.65742.)	The mCherry sequence was inserted via Crispr/Cas9 in the rin locus, at the N-terminus of the rin coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mCherry. Rin is the Drosophila ortholog of the G3BP1; it is Stress Granule marker positive.	rasputin	N-terminal mCherry tag Stress Granule marker mCherry knock-in	Sign In to show prices
310603	310603	FB-STOCK	tagged construct	w*; ; Patr-1[KI.mTomato]	The mTomato sequence was inserted via Crispr/Cas9 in the Patr-1 locus, at the C-terminus of the Patr-1 coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. Patr-1 is the Drosophila ortholog of PATL1; it can be used as a P-body marker.	310603	Pushpalatha et al., Nat com (2022)	Florence Besse	Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)	Patr-1	BcDNA:LD27979 C-terminal mTomato tag HPat HPat1 Hpat LD27979 P-body marker Part-1 Patr-1 Protein associated with topo II related - 1 anon-WO0118547.282 hpat knock-in l(3)06442 lethal (3) 06442 mTomato patr-1	see publication (Formicola et al., 2021; doi: 10.7554/eLife.65742.)	The mTomato sequence was inserted via Crispr/Cas9 in the Patr-1 locus, at the C-terminus of the Patr-1 coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. Patr-1 is the Drosophila ortholog of PATL1; it can be used as a P-body marker.	Protein associated with topo II related - 1	P-body marker knock-in C-terminal mTomato tag mTomato	Sign In to show prices
310600	310600	FB-STOCK	tagged construct	w*; me31B[KI.EGFP]	The EGFP sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae EGFP positive. Me31B is the Drosophila ortholog of DDX-6; it is a P-body marker.	310600	Formicola et al., eLife (2021)	Florence Besse	Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)	me31B	C-terminal EGFP tag DDX6 Dhh1p/Me31b DmRH3 EGFP ME31B Mat31B Maternal expression at 31B Me31B Me31b P-body marker knock-in l(2)k06607 maternal expression at 31B me31B me31b	see publication	The EGFP sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae EGFP positive. Me31B is the Drosophila ortholog of DDX-6; it is a P-body marker.	maternal expression at 31B	knock-in EGFP C-terminal EGFP tag P-body marker	Sign In to show prices