KEGG ENZYME: 4.2.1.36

ENZYME: 4.2.1.36

Entry

EC 4.2.1.36                 Enzyme

Name

homoaconitate hydratase;
homoaconitase;
cis-homoaconitase;
HACN;
Lys4;
LysF;
2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase (incorrect)

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(Z)-but-1-ene-1,2,4-tricarboxylate-forming]

Reaction(IUBMB)

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O [RN:R04371]

Reaction(KEGG)

R04371

Substrate

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [CPD:C05662]

Product

(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:C04002];
H2O [CPD:C00001]

Comment

Requires a [4Fe-4S] cluster for activity. The enzyme from the hyperthermophilic eubacterium Thermus thermophilus can catalyse the reaction shown above but cannot catalyse the previously described reaction, i.e. formation of (R)-homocitrate by hydration of cis-homoaconitate. The enzyme responsible for the conversion of cis-homoaconitate into (R)-homocitrate in T. thermophilus is unknown at present but the reaction can be catalysed in vitro using aconitate hydratase from pig (EC 4.2.1.3) [2].

History

EC 4.2.1.36 created 1972, modified 2007

Pathway

ec00300

Lysine biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

ec01120

Microbial metabolism in diverse environments

Orthology

K01705

homoaconitate hydratase

Genes

SCE:	YDR234W(LYS4)

SEUB:

DI49_0416

SPAO:

SPAR_D04320

AGO:	AGOS_ABL106C

ERC:

Ecym_5123

KLA:	KLLA0_C15125g

KMX:	KLMA_30527(LYS4)

LTH:	KLTH0E10582g

VPO:	Kpol_1031p57

ZRO:	ZYRO0A10076g

CGR:	2890081(GVI51_K10813)

NCS:	NCAS_0B02660(NCAS0B02660)

NDI:	NDAI_0C05030(NDAI0C05030)

TPF:	TPHA_0L01150(TPHA0L01150)

TBL:	TBLA_0A01460(TBLA0A01460)

TGB:	HG536_0H01840

TDL:	TDEL_0B01800(TDEL0B01800)

KAF:	KAFR_0E02730(KAFR0E02730)

KNG:	KNAG_0A04240(KNAG0A04240)

ZMK:	HG535_0F05020

SLUD:

SCDLUD_001925

DHA:	DEHA2F07238g

PIC:	PICST_88705(LYS4)

PGU:	PGUG_01420

SPAA:

SPAPADRAFT_58782(LYS4)

LEL:	PVL30_004416(LYS4)

LBG:	92209228(LODBEIA_P40320)

CAL:	CAALFM_C404410CA(LYS4)

CTP:	CTRG_03911

COT:	CORT_0B00840

CDU:	CD36_44080

CTEN:

18246180(LYS4)

CLU:	CLUG_00676

CLUS:

A9F13_17g00583

CAUR:

CJI96_0000218

PPA:	PAS_chr4_0795

PKZ:	C5L36_0B00600

BNN:	FOA43_001879(LYS4)

BBRX:

BRETT_004628(LYS4)

OPA:	HPODL_02077

YLI:	2912118(YALI2_E01707g)

SLB:	AWJ20_348(LYS4)

NCR:	NCU08898(lys-6)

NTE:	NEUTE1DRAFT58933(NEUTE1DRAFT_58933)

SMP:	10804255(SMAC4_05116)

PAN:	PODANSg8469

PBEL:

QC761_206510(LYS4)

PPSD:

QC762_0038220(LYS4)

PPSP:

QC763_206510(LYS4)

PPSA:

QC764_206510(LYS4)

TTT:	THITE_2124288

MTM:	MYCTH_2301648

CTHR:

CTHT_0069590

MGR:

MGG_04842

PPEI:

PpBr36_03207

PGRI:

PgNI_01490

TMN:	UCRPA7_611

SSCK:

SPSK_03952

FGR:	FGSG_10949

FPU:	FPSE_06063

FPOA:

FPOAC1_009694(LYS4) FPOAC1_012803

FVN:	FVRRES_10333

FVR:	FVEG_11644

FOX:	FOXG_12892

NHE:	NECHADRAFT_103327 NECHADRAFT_78315 NECHADRAFT_95300

FFC:	NCS54_01005400

FKR:	NCS57_00218100 NCS57_00999500

FMU:	J7337_010041(LYS4)

TRE:	TRIREDRAFT_81570

TRR:	M419DRAFT_142874

TASP:

36612066(LYS4)

MAW:	19251867(LYS4_3) 19253319(LYS4_1)

MAJ:	MAA_04686 MAA_09361

MBRN:

26240367(LYS4_1) 26243995(LYS4_0)

PCHM:

VFPPC_00805

CMT:

CCM_07793

AMUS:

LMH87_002766

PLJ:	28892921(PLICBS_009087)

PTKZ:

JDV02_004274(LYS4)

VAL:	VDBG_00257

VDA:	VDAG_08540

CFJ:	CFIO01_08629

CLUP:

CLUP02_07655

CHIG:

CH63R_04010 CH63R_10446

CDET:

87941043(CDEST_04540) 87943557(CDEST_07054)

SAPO:

SAPIO_CDS2378 SAPIO_CDS2954

ELA:	UCREL1_5880

PFY:	PFICI_04889

SSL:	SS1G_01026

BFU:	BCIN_14g00430(Bclys4)

MBE:

MBM_05638

PSCO:

LY89DRAFT_738951

GLZ:	GLAREA_01173

ANI:	ANIA_06521

AFM:	AFUA_5G08890

ACT:	ACLA_011660

NFI:	NFIA_078420

AOR:	AO090701000041

ANG:	An15g00350

AFV:	AFLA_004530

ALUC:

AKAW2_60650A(LYS4)

ACHE:

ACHE_50239A(LYS4)

APUU:

APUU_10764A(LYS4)

PCS:	N7525_011096

PDP:	PDIP_05800

POU:	POX_c04492

TMF:	EYB26_009661

TRG:	TRUGW13939_08882

CIM:	CIMG_02639

CPW:	9696652(LYS4)

URE:	UREG_03117

ABE:

ARB_04155

TVE:

TRV_03936

PBL:	PAAG_03612

PBN:	PADG_06215

AJE:	HCAG_06854

BGH:	BDBG_09384

PNO:	SNOG_01131(SNOG_01129 SNOG_01130)

PTE:

PTT_19520

PTRR:

6341958(PtrM4_095890)

BZE:	COCCADRAFT_82932

BSC:	COCSADRAFT_152920

BOR:	COCMIDRAFT_86014

AALT:

CC77DRAFT_1042638

ARAB:

EKO05_0008657

ZTR:	MYCGRDRAFT_68535

PFJ:	MYCFIDRAFT_202095

FFU:	CLAFUR5_03230

CBET:

CB0940_03357

BCOM:

BAUCODRAFT_34146

NPA:	UCRNP2_10443

TML:	GSTUM_00011608001

SPO:	2542867(lys2)

SOM:	SOMG_02039(lys2)

CNE:

CNK00580

CNB:

CNBK2910

CNG:	CNAG_02565

CGI:	CGB_K3700C

CDEU:

CNBG_3213

CDEP:

91088695(L203_104485)

KMG:	30164303(I203_106012)

KNE:	92183516(IAR55_006258)

TMS:	TREMEDRAFT_44947

TASA:

A1Q1_04925

CCAC:

CcaHIS019_0702660(LYS4)

TVS:	TRAVEDRAFT_150870

DSQ:	DICSQDRAFT_155148

PCO:	PHACADRAFT_252820

SHS:	STEHIDRAFT_72757

HIR:	HETIRDRAFT_314980

PSQ:	PUNSTDRAFT_66471

ADL:	AURDEDRAFT_112541

FME:	FOMMEDRAFT_76165

GTR:	GLOTRDRAFT_107515

RSX:	RhiXN_03086

LBC:	LACBIDRAFT_184939

CCI:	CC1G_14391

ABP:	AGABI1DRAFT59676(AGABI1DRAFT_59676)

ABV:	AGABI2DRAFT184036(AGABI2DRAFT_184036)

PCUB:

JR316_0006214

MRR:	Moror_4605

E1B28_011496(LYS4_2)

SCM:	SCHCO_02541718(SCHCODRAFT_02541718)

CPUT:

CONPUDRAFT_119840

SLA:	SERLADRAFT_468242

WSE:	WALSEDRAFT_59020

WIC:	J056_004489

UMA:	UMAG_10689

PFP:	PFL1_04410

SGRA:

EX895_000383

MGL:

MGL_3298

MRT:

MRET_2395

MSYM:

MSY001_3108

PGR:	PGTG_09399

PTRC:

PtA15_8A618

PSTR:

Pst134EA_025611

MLR:	MELLADRAFT_105205

RIE:	OCT59_029567

MBR:	MONBRDRAFT_32374

SRE:	PTSG_09627

ACAN:

ACA1_061650

PIF:	PITG_12462

PSOJ:

PHYSODRAFT_562967

SPAR:

SPRG_03993

NGR:	NAEGRDRAFT_80875

CALL:

OZ401_000503(lysF)

UAM:	UABAM_01973

ABA:	Acid345_2965

IAL:

IALB_1150

MRO:

MROS_2220

CABY:

Cabys_582

» show all

Reference

1 [PMID:5954805]

Authors

Strassman M, Ceci LN.

Title

Enzymatic formation of cis-homoaconitic acid, an intermediate in lysine biosynthesis in yeast.

Journal

J Biol Chem 241:5401-7 (1966)

Reference

2 [PMID:16524361]

Authors

Jia Y, Tomita T, Yamauchi K, Nishiyama M, Palmer DR.

Title

Kinetics and product analysis of the reaction catalysed by recombinant homoaconitase from Thermus thermophilus.

Journal

Biochem J 396:479-85 (2006)
DOI:10.1042/BJ20051711

Reference

3 [PMID:10714900]

Authors

Zabriskie TM, Jackson MD.

Title

Lysine biosynthesis and metabolism in fungi.

Journal

Nat Prod Rep 17:85-97 (2000)
DOI:10.1039/A801345D

Other DBs

ExplorEnz - The Enzyme Database:

4.2.1.36

IUBMB Enzyme Nomenclature:

4.2.1.36

ExPASy - ENZYME nomenclature database:

4.2.1.36

BRENDA, the Enzyme Database:

4.2.1.36

CAS:

9030-68-6

LinkDB

DBGET integrated database retrieval system