Lactobacillus amylovorus GRL1118: LAB52_02440
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Entry
LAB52_02440 CDS
T01954
Name
(GenBank) thermostable pullulanase
KO
K01200
pullulanase [EC:
3.2.1.41
]
Organism
lay
Lactobacillus amylovorus GRL1118
Pathway
lay00500
Starch and sucrose metabolism
lay01100
Metabolic pathways
lay01110
Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:
lay00001
]
09100 Metabolism
09101 Carbohydrate metabolism
00500 Starch and sucrose metabolism
LAB52_02440
Enzymes [BR:
lay01000
]
3. Hydrolases
3.2 Glycosylases
3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
3.2.1.41 pullulanase
LAB52_02440
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Gene cluster
GFIT
Motif
Pfam:
SlpA
Alpha-amylase
PUD
CBM_48
GlgB_N
Pullul_strch_C
PFF1_TM
Motif
Other DBs
NCBI-ProteinID:
AEA31469
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Position
477457..481041
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AA seq
1194 aa
AA seq
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MNKNKNRYSRSKCVFVCASAILSLWLGATWTNNVKAAEVDANQAANNTEQTQQNTDHVTA
NKQDKTKDVDNQATPTATEQNNAQPAANAKSSDSASATPQSDTETVKNTTKVVVHYQGDG
TKWVPYVWGKNPNSTGVQYNWDGQDNYGYYANITVDNDEQKVGVLIKGTDSWDKDGTGAD
RSLTVDDNGKAEVWYKEGSDDSQDVTPSYNSAKIKVHYHGNTDATSLNYWTDTDQSKKAV
SLSKEDDDDEIGDFDLTGQTFSKIYVSPIGSDTETREFTPLPGDGTTDIYLVNKDTTAYY
TPSFALAPESLASASMESRNTVDIKTAKKMTADEAKAILQMKDNEVVDVSAVDADQDGKS
KEFKITTAKDLDILTNNQIGIYNNFRSIDIGKYVRSDEFDAKYYYGGDDLGATYTQAQTQ
IKLWAPTAKKVVLNLYASLDNDSTATKTFVMTRGDKGVWSVSLPGDYKNWAYDYSLTFGD
GSETKSNDPYSKAVTINGDRSVIEDYDAIKPDDFNRMPSFSSPTNAIIYETSIRDFTSDP
NSGIKDKGKYLGMIESGVAPDGQVTGLDYLKSLGITHVQIMPMFDFASIDETKNDGTYNW
GYDPKNYNVPEGSYSSNAANPTARIMEMKEMINGLHKAGIRVIMDVVYNHVYSPNDQALQ
KTVPGYYFEYDDDNNMVADSGCGNDTASERLMMRKYILDSVKYWAKNYNIDGFRFDIMSL
LDTTTMNDIRDELNQIDPGMLLYGEGWDMRTTKREIGAGQYNADKIPSIGFFSDDIRNSV
RSDEPNYAPGLVIGDGKEEKYEADAKKFIASYLGGKDLAIPGVTWQIHPYQSPSQTINYV
ACHDNRTLYDLLKWALPDESEANIIKRDKLATSMIMLAEGVPFVYGGQEALRTKDGNNNS
YNAPIEVNEINWERVKDNQDLVNYFKELVNVRKSQPALRLNNYDEIAKTVKTVNAGEGGI
FTFAYKEDGKTLYVVFNVNDSNAELNDVDLSKADKLLDSDGNVVLGKTTTLMPLSTLIAV
VPDETPVTPETPSDTGSITNTGSADSSQIQGNGDTDKLLPALDIVLTHNAYIYQNDGITT
LKENGKNIVLKLGKTIHAMNNGQIFTFNGKKFYQIGENEYIKVNNTLKRKVLIHNAFVYT
KSGKAIKKGHKHIVLKKKSTILALDNDKIVTIKGKKFYRIGNNKYVKVANVKKD
NT seq
3585 nt
NT seq
+upstream
nt +downstream
nt
atgaataaaaataaaaaccgttacagtagaagtaaatgcgtatttgtttgtgcatcagcc
attttgagcctttggcttggtgcaacttggactaacaatgttaaagccgctgaagttgac
gcaaatcaagcagcaaataacaccgaacaaacacagcaaaatacagatcatgtaacggct
aataaacaagataaaacaaaagatgtagataatcaagctacacctactgcaactgagcaa
aataatgcgcaaccagcagcaaatgcaaaaagtagtgattcagcttctgcaacacctcaa
tctgatacagaaacggtaaagaacacaactaaagttgttgttcactatcaaggtgatggt
actaaatgggtaccttatgtatggggcaagaatccaaacagtactggtgttcaatataat
tgggacggccaggataattatggttattatgccaacattacagtcgataatgatgaacaa
aaagtaggagttttgattaaaggtacagactcctgggataaagatggtacaggtgccgac
cgttcattgactgttgacgataatggtaaggctgaagtttggtacaaagaaggttctgat
gatagtcaggatgtaactccaagctataattcagctaaaataaaagttcattatcatggt
aatactgatgcaacaagtcttaattactggactgatactgatcaaagtaaaaaggcagta
tcattatcgaaggaagacgatgatgatgaaattggtgactttgatttgacgggccaaaca
tttagcaagatctatgtttcaccaattggtagtgatactgagactagagaatttactcca
cttccaggtgatggaactactgatatttatttagtaaataaagatacaacggcttactac
acaccaagctttgctttagcaccagaaagcttagcttcagcttcaatggaatcacgcaat
acagtagatataaagactgctaaaaagatgaccgcagatgaagctaaagccatcttacaa
atgaaggataatgaagttgttgatgtatccgcagttgacgctgatcaggatggcaagagt
aaagaatttaagattaccacagctaaagatcttgatattttaactaataatcaaattgga
atttacaacaactttaggtctatcgatattggcaaatacgtcagatctgatgaattcgat
gccaaatattactatggcggcgatgatttaggtgcaacttacactcaagcacaaacccaa
attaaactttgggcaccaacagctaagaaagttgttttgaacttatatgcctcattagat
aatgactcaactgcaactaagacatttgtcatgactcgtggtgataagggcgtttggtca
gtatctcttccaggtgattacaagaattgggcatatgattacagcttaaccttcggtgat
ggtagcgagactaaaagtaatgatccatactcaaaggcagtaaccatcaatggtgatcgt
tcagttattgaagattatgatgcaattaaaccagatgactttaatagaatgccttcattc
tcaagcccaactaatgcaattatctatgaaaccagcattcgtgactttacgagtgatcca
aactcaggtattaaagataaaggtaaatatttaggcatgattgaatcaggcgtagctcct
gatggccaggtcactggtttggattacttgaagtcattaggcattactcatgttcaaatt
atgccaatgtttgacttcgcaagtattgatgaaactaagaatgatggaacttataattgg
ggttatgatcctaagaactataatgttccagaaggaagctattcaagcaacgcagctaat
ccaaccgcaagaatcatggaaatgaaagaaatgatcaacggtttgcacaaggctggtatt
agagtaatcatggatgttgtttacaaccacgtatactcaccaaacgaccaggccttgcaa
aagacagttccaggttattacttcgaatacgatgatgataacaatatggtagctgatagt
ggttgtggtaatgatactgccagtgaacgtctgatgatgagaaagtatatcctggattca
gttaaatactgggctaaaaactacaacattgatggcttccgctttgatattatgtcattg
cttgatactactaccatgaatgatattcgggatgaattaaatcagattgatcctggtatg
cttctttatggtgaaggctgggatatgagaaccaccaagcgtgagatcggtgctggtcaa
tacaacgcagataaaatcccttcaattggcttcttcagtgatgatattagaaactcagta
agatctgatgaaccaaattacgctccaggtttggttatcggtgatggaaaagaagaaaag
tatgaagctgatgctaagaagtttattgccagctacttgggcggtaaggacttagcaatt
cctggtgttacttggcagattcacccataccaatcaccaagtcaaactatcaactatgtt
gcttgtcacgataaccgtactttgtatgacttgttgaagtgggctttacctgatgaatct
gaagctaatattattaagcgtgataaattagcaacgtcaatgatcatgttagcagaaggt
gtgccatttgtttatggcggccaagaagctttaagaactaaagatggtaacaataatagt
tacaatgctccaattgaagtcaacgaaattaattgggaacgtgtcaaagacaatcaagat
cttgttaactacttcaaagaattagttaatgtacgtaagagtcaacctgccttaagattg
aacaattatgatgaaatcgctaagacagtaaagacagtcaatgctggtgaaggtggtatc
tttactttcgcttacaaagaagatggcaagacactttatgtagtatttaatgttaacgat
agtaatgctgaattgaatgatgttgatttgtctaaagctgataagttgctagacagcgat
ggaaatgttgtcttaggcaagactacaacattgatgccacttagcacgttgattgctgta
gttccagatgaaactccagtaaccccggaaacccctagtgatactggctccatcactaat
actggttcagctgattcatcacaaattcaaggcaatggtgatactgataagcttttacca
gctcttgatattgttttgactcataatgcttatatctatcaaaatgatggaattactact
cttaaagaaaatggtaagaacattgtccttaaattgggtaaaactattcatgctatgaac
aatggtcaaatctttaccttcaatggtaagaaattctaccaaattggagaaaatgaatac
atcaaagttaataatactttgaaacgcaaagttttgatccataacgcttttgtttacaca
aagagtggtaaggccattaagaaaggtcacaagcatattgttctgaagaagaagagcaca
atcttagcactggataatgataagattgttactatcaaaggtaaaaagttctatcgcatt
ggtaacaataaatatgtcaaagttgctaatgttaaaaaggattaa
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