Galphao G protein alpha subunit o [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Galphaoprovided by FlyBase
Official Full Name: G protein alpha subunit oprovided by FlyBase
Primary source: FLYBASE:FBgn0001122
Locus tag: Dmel_CG2204
See related: AllianceGenome:FB:FBgn0001122
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: alpha(o); bkh; Bkh; CG2204; DGalpha0; dGalphao; DGalpha[[o]]; dgo; dgoalpha; DG[[o]]; Dmel\CG2204; drogpad; DROGPAD; G(o)alpha47A; G-alpha-47A; G-oalpha; G-oalpha47a; G-oalpha47A; Galpha47A; Galphai/o; Galphao47A; Galpha[[047A]]; Galpha[[0]]; Galpha[[o]]; Galpha[[o]]47A; Go; Go-alpha; Goa; goalpha; Goalpha; Goalpha-47A; Goalpha47a; G[[oalpha]]; G[[o]]; G[[o]]alpha; like GNAO
Summary: Enables GTP binding activity. Involved in several processes, including behavioral response to starvation; negative regulation of synaptic assembly at neuromuscular junction; and nervous system development. Located in plasma membrane. Part of heterotrimeric G-protein complex. Is expressed in several structures, including female germline cell; ganglia; organism subdivision; somatic precursor cell; and visceral mesoderm derivative. Used to study developmental and epileptic encephalopathy 17. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 17 and neurodevelopmental disorder with involuntary movements. Orthologous to human GNAO1 (G protein subunit alpha o1). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: all
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Genomic context

Location:: 47A7-47A9; 2-62 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (10437985..10465701)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (6325490..6353206)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Neomorphic Galphao mutations gain interaction with Ric8 proteins in GNAO1 encephalopathies.
Title: Neomorphic Gαo mutations gain interaction with Ric8 proteins in GNAO1 encephalopathies.
We have taken advantage of this approach to stabilize protein G alpha-go from Drosophila melanogaster. The designed SS-bridge increased by 4 degrees the melting temperature of one domain of protein G alpha-go .
Title: Intrinsic Disorder-Based Design of Stabilizing Disulphide Bridge in Gαo Protein.
The heterotrimeric G protein Go functions as a transducer of Wingless-Frizzled 2 signaling in the synapse and Ankyrin 2 as a target of Go signaling required for neuromuscular junction formation.
Title: Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton.
Data indicate that Kermit interacts with Go and another PCP component Vang during planar cell polarity (PCP).
Title: Kermit interacts with Gαo, Vang, and motor proteins in Drosophila planar cell polarity.
Galphao47A is required in bitter-sensitive taste neurons for L-canavanine sensitivity.
Title: Gαo is required for L-canavanine detection in Drosophila.
Go is required for maximal physiological responses to multiple odorants in Drosophila, and olfactory receptor channel function and G-protein signaling are required for odor responsiveness.
Title: Go contributes to olfactory reception in Drosophila melanogaster.
Study shows that G-oalpha47A and Gbeta13F cooperate with Ggamma1 to maintain cardiac integrity in Drosophila.
Title: Heterotrimeric G proteins regulate a noncanonical function of septate junction proteins to maintain cardiac integrity in Drosophila.
The roles for Galpha(o) subunit (Go) in mediating the two distinct pathways transduced by Fz receptors in Drosophila.
Title: Trimeric G protein-dependent frizzled signaling in Drosophila.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein beta/gamma-subunit complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein beta/gamma-subunit complex binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in asymmetric cell division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon ensheathment in central nervous system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in behavioral response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcium-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell adhesion involved in heart morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cortical actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of glial blood-brain barrier	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of imaginal disc-derived wing hair orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heart development	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of synaptic assembly at neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in non-canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory perception of sweet taste	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in septate junction assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ventral cord development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of heterotrimeric G-protein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of heterotrimeric G-protein complex	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: G protein alpha subunit o

Names: CG2204-PA; CG2204-PB; CG2204-PC; CG2204-PD; CG2204-PE; CG2204-PF; CG2204-PG; CG2204-PH; CG2204-PI; CG2204-PJ; G protein alpha o subunit; G protein o alpha 47A; G protein oalpha 47A; Galphao-PA; Galphao-PB; Galphao-PC; Galphao-PD; Galphao-PE; Galphao-PF; Galphao-PG; Galphao-PH; Galphao-PI; Galphao-PJ; brokenheart; brokenhearted; drogpad

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

10437985..10465701

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_165772.4 → NP_724934.1 G protein alpha subunit o, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724934.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P16378

Related

FBpp0087359

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
NM_165773.3 → NP_724935.1 G protein alpha subunit o, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724935.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZB6, A4UZC1, B7FF70, P16377, P16378, P16707, Q540V8, Q8IGI5, Q9V5L5, Q9V5L6

Related

FBpp0087360

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
NM_176125.3 → NP_788305.1 G protein alpha subunit o, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788305.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P16378

Related

FBpp0087363

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
NM_176124.3 → NP_788304.1 G protein alpha subunit o, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788304.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P16378

Related

FBpp0087362

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
NM_176126.3 → NP_788306.1 G protein alpha subunit o, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788306.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P16378

Related

FBpp0087364

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
NM_176127.3 → NP_788307.1 G protein alpha subunit o, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788307.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P16378

Related

FBpp0087365

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
NM_206080.2 → NP_995802.1 G protein alpha subunit o, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995802.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P16378

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
NM_078960.5 → NP_523684.2 G protein alpha subunit o, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523684.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZB6, A4UZC1, B7FF70, P16377, P16378, P16707, Q540V8, Q8IGI5, Q9V5L5, Q9V5L6

Related

FBpp0087361

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
NM_206079.2 → NP_995801.1 G protein alpha subunit o, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995801.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZB6, A4UZC1, B7FF70, P16377, P16378, P16707, Q540V8, Q8IGI5, Q9V5L5, Q9V5L6

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
NM_001273940.1 → NP_001260869.1 G protein alpha subunit o, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260869.1

Status: REVIEWED

UniProtKB/TrEMBL

D5AEN0

Conserved Domains (1) summary

cd00066
Location:1 → 121

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)