Structural insights into the diversity and DNA cleavage mechanism of Fanzor.
Cell.
2024 Sep 19;187(19):5238-5252.e20. doi: 10.1016/j.cell.2024.07.050. Epub 2024 Aug 28. PubMed PMID:
39208796; PubMed Central PMCID:
PMC11423790.
Structural determinants of DNA cleavage by a CRISPR HNH-Cascade system.
Mol Cell.
2024 Aug 22;84(16):3154-3162.e5. doi: 10.1016/j.molcel.2024.07.026. Epub 2024 Aug 6. PubMed PMID:
39111310; PubMed Central PMCID:
PMC11459484.
Internal initiation of reverse transcription in a Penelope-like retrotransposon.
Mob DNA.
2024 Jun 11;15(1):12. doi: 10.1186/s13100-024-00322-z. PubMed PMID:
38863000; PubMed Central PMCID:
PMC11167929.
Human paraneoplastic antigen Ma2 (PNMA2) forms icosahedral capsids that can be engineered for mRNA delivery.
Proc Natl Acad Sci U S A.
2024 Mar 12;121(11):e2307812120. doi: 10.1073/pnas.2307812120. Epub 2024 Mar 4. PubMed PMID:
38437549; PubMed Central PMCID:
PMC10945824.
Diversity, evolution, and classification of the RNA-guided nucleases TnpB and Cas12.
Proc Natl Acad Sci U S A.
2023 Nov 28;120(48):e2308224120. doi: 10.1073/pnas.2308224120. Epub 2023 Nov 20. PubMed PMID:
37983496; PubMed Central PMCID:
PMC10691335.
Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering.
Science.
2023 Nov 24;382(6673):eadi1910. doi: 10.1126/science.adi1910. Epub 2023 Nov 23. PubMed PMID:
37995242; PubMed Central PMCID:
PMC10910872.
Drug delivery systems for CRISPR-based genome editors.
Nat Rev Drug Discov.
2023 Nov;22(11):875-894. doi: 10.1038/s41573-023-00762-x. Epub 2023 Sep 18. Review. PubMed PMID:
37723222.
Cell type-specific delivery by modular envelope design.
Nat Commun.
2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8. PubMed PMID:
37612276; PubMed Central PMCID:
PMC10447438.
Exposure of iPSC-derived human microglia to brain substrates enables the generation and manipulation of diverse transcriptional states in vitro.
Nat Immunol.
2023 Aug;24(8):1382-1390. doi: 10.1038/s41590-023-01558-2. Epub 2023 Jul 27. PubMed PMID:
37500887; PubMed Central PMCID:
PMC10382323.
Fanzor is a eukaryotic programmable RNA-guided endonuclease.
Nature.
2023 Aug;620(7974):660-668. doi: 10.1038/s41586-023-06356-2. Epub 2023 Jun 28. PubMed PMID:
37380027; PubMed Central PMCID:
PMC10432273.
Multicellular communities are perturbed in the aging human brain and Alzheimer's disease.
Nat Neurosci.
2023 Jul;26(7):1267-1280. doi: 10.1038/s41593-023-01356-x. Epub 2023 Jun 19. PubMed PMID:
37336975; PubMed Central PMCID:
PMC10789499.
Modularity and diversity of target selectors in Tn7 transposons.
Mol Cell.
2023 Jun 15;83(12):2122-2136.e10. doi: 10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1. PubMed PMID:
37267947; PubMed Central PMCID:
PMC10293859.
The Transposon-Encoded Protein TnpB Processes Its Own mRNA into ωRNA for Guided Nuclease Activity.
CRISPR J.
2023 Jun;6(3):232-242. doi: 10.1089/crispr.2023.0015. PubMed PMID:
37272862; PubMed Central PMCID:
PMC10278001.
Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription.
Science.
2023 Apr 21;380(6642):301-308. doi: 10.1126/science.adg7883. Epub 2023 Apr 6. PubMed PMID:
37023171; PubMed Central PMCID:
PMC10499050.
Cryo-EM structure of the transposon-associated TnpB enzyme.
Nature.
2023 Apr;616(7956):390-397. doi: 10.1038/s41586-023-05933-9. Epub 2023 Apr 5. PubMed PMID:
37020030; PubMed Central PMCID:
PMC10097598.
Programmable protein delivery with a bacterial contractile injection system.
Nature.
2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. PubMed PMID:
36991127; PubMed Central PMCID:
PMC10097599.
A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance.
CRISPR J.
2023 Apr;6(2):99-115. doi: 10.1089/crispr.2022.0048. Epub 2022 Nov 11. PubMed PMID:
36367987; PubMed Central PMCID:
PMC7614457.
A transcription factor atlas of directed differentiation.
Cell.
2023 Jan 5;186(1):209-229.e26. doi: 10.1016/j.cell.2022.11.026. PubMed PMID:
36608654; PubMed Central PMCID:
PMC10344468.
RNA-activated protein cleavage with a CRISPR-associated endopeptidase.
Science.
2022 Nov 25;378(6622):874-881. doi: 10.1126/science.add7450. Epub 2022 Nov 3. PubMed PMID:
36423276; PubMed Central PMCID:
PMC10028731.
Structure of the IscB-ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9.
Nat Commun.
2022 Nov 7;13(1):6719. doi: 10.1038/s41467-022-34378-3. PubMed PMID:
36344504; PubMed Central PMCID:
PMC9640706.
Structure of the OMEGA nickase IsrB in complex with ωRNA and target DNA.
Nature.
2022 Oct;610(7932):575-581. doi: 10.1038/s41586-022-05324-6. Epub 2022 Oct 12. PubMed PMID:
36224386; PubMed Central PMCID:
PMC9581776.
Microfluidic Enrichment and Computational Analysis of Rare Sequences from Mixed Genomic Samples for Metagenomic Mining.
CRISPR J.
2022 Oct;5(5):677-684. doi: 10.1089/crispr.2022.0054. Epub 2022 Oct 7. PubMed PMID:
36206017; PubMed Central PMCID:
PMC9595627.
Structure and engineering of the minimal type VI CRISPR-Cas13bt3.
Mol Cell.
2022 Sep 1;82(17):3178-3192.e5. doi: 10.1016/j.molcel.2022.08.001. Epub 2022 Aug 25. PubMed PMID:
36027912; PubMed Central PMCID:
PMC7613696.
Prokaryotic innate immunity through pattern recognition of conserved viral proteins.
Science.
2022 Aug 12;377(6607):eabm4096. doi: 10.1126/science.abm4096. Epub 2022 Aug 12. PubMed PMID:
35951700; PubMed Central PMCID:
PMC10028730.
Neurotensin orchestrates valence assignment in the amygdala.
Nature.
2022 Aug;608(7923):586-592. doi: 10.1038/s41586-022-04964-y. Epub 2022 Jul 20. PubMed PMID:
35859170; PubMed Central PMCID:
PMC9583860.
UG/Abi: a highly diverse family of prokaryotic reverse transcriptases associated with defense functions.
Nucleic Acids Res.
2022 Jun 24;50(11):6084-6101. doi: 10.1093/nar/gkac467. PubMed PMID:
35648479; PubMed Central PMCID:
PMC9226505.
Human pathogenic RNA viruses establish noncompeting lineages by occupying independent niches.
Proc Natl Acad Sci U S A.
2022 Jun 7;119(23):e2121335119. doi: 10.1073/pnas.2121335119. Epub 2022 May 31. PubMed PMID:
35639694; PubMed Central PMCID:
PMC9191635.
Epistasis at the SARS-CoV-2 Receptor-Binding Domain Interface and the Propitiously Boring Implications for Vaccine Escape.
mBio.
2022 Apr 26;13(2):e0013522. doi: 10.1128/mbio.00135-22. Epub 2022 Mar 15. PubMed PMID:
35289643; PubMed Central PMCID:
PMC9040817.
CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity.
Nat Commun.
2022 Mar 25;13(1):1606. doi: 10.1038/s41467-022-29205-8. PubMed PMID:
35338135; PubMed Central PMCID:
PMC8956604.
Compact RNA editors with small Cas13 proteins.
Nat Biotechnol.
2022 Feb;40(2):194-197. doi: 10.1038/s41587-021-01030-2. Epub 2021 Aug 30. PubMed PMID:
34462587; PubMed Central PMCID:
PMC8929162.
Cargo Genes of Tn7-Like Transposons Comprise an Enormous Diversity of Defense Systems, Mobile Genetic Elements, and Antibiotic Resistance Genes.
mBio.
2021 Dec 21;12(6):e0293821. doi: 10.1128/mBio.02938-21. Epub 2021 Dec 7. PubMed PMID:
34872347; PubMed Central PMCID:
PMC8649781.
LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding.
Nat Biotechnol.
2021 Dec;39(12):1556-1562. doi: 10.1038/s41587-021-00966-9. Epub 2021 Jun 29. PubMed PMID:
34188222; PubMed Central PMCID:
PMC8678193.
The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases.
Science.
2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. PubMed PMID:
34591643; PubMed Central PMCID:
PMC8929163.
Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery.
Science.
2021 Aug 20;373(6557):882-889. doi: 10.1126/science.abg6155. PubMed PMID:
34413232; PubMed Central PMCID:
PMC8431961.
Ongoing global and regional adaptive evolution of SARS-CoV-2.
Proc Natl Acad Sci U S A.
2021 Jul 20;118(29). doi: 10.1073/pnas.2104241118. Epub 2021 Jul 2. PubMed PMID:
34292871; PubMed Central PMCID:
PMC8307621.
A cellular and spatial map of the choroid plexus across brain ventricles and ages.
Cell.
2021 May 27;184(11):3056-3074.e21. doi: 10.1016/j.cell.2021.04.003. Epub 2021 Apr 30. PubMed PMID:
33932339; PubMed Central PMCID:
PMC8214809.
Dual modes of CRISPR-associated transposon homing.
Cell.
2021 Apr 29;184(9):2441-2453.e18. doi: 10.1016/j.cell.2021.03.006. Epub 2021 Mar 25. PubMed PMID:
33770501; PubMed Central PMCID:
PMC8276595.
Genome-wide CRISPR screens reveal synthetic lethal interaction between CREBBP and EP300 in diffuse large B-cell lymphoma.
Cell Death Dis.
2021 Apr 28;12(5):419. doi: 10.1038/s41419-021-03695-8. PubMed PMID:
33911074; PubMed Central PMCID:
PMC8080727.
Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3.
Proc Natl Acad Sci U S A.
2021 Mar 9;118(10). doi: 10.1073/pnas.2020401118. PubMed PMID:
33649229; PubMed Central PMCID:
PMC7958351.
Pluripotent stem cell-derived models of neurological diseases reveal early transcriptional heterogeneity.
Genome Biol.
2021 Mar 4;22(1):73. doi: 10.1186/s13059-021-02301-6. PubMed PMID:
33663567; PubMed Central PMCID:
PMC7934477.
Regulation of purine metabolism connects KCTD13 to a metabolic disorder with autistic features.
iScience.
2021 Jan 22;24(1):101935. doi: 10.1016/j.isci.2020.101935. eCollection 2021 Jan 22. PubMed PMID:
33409479; PubMed Central PMCID:
PMC7773955.
Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA.
Nat Biomed Eng.
2020 Dec;4(12):1140-1149. doi: 10.1038/s41551-020-00603-x. Epub 2020 Aug 26. PubMed PMID:
32848209.
In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes.
Science.
2020 Nov 27;370(6520). doi: 10.1126/science.aaz6063. PubMed PMID:
33243861; PubMed Central PMCID:
PMC7985844.
Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay.
PLoS One.
2020;15(11):e0238612. doi: 10.1371/journal.pone.0238612. eCollection 2020. PubMed PMID:
33137122; PubMed Central PMCID:
PMC7605681.
Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing.
N Engl J Med.
2020 Oct 8;383(15):1492-1494. doi: 10.1056/NEJMc2026172. Epub 2020 Sep 16. PubMed PMID:
32937062; PubMed Central PMCID:
PMC7510942.
A Survey of Genome Editing Activity for 16 Cas12a Orthologs.
Keio J Med.
2020 Sep 25;69(3):59-65. doi: 10.2302/kjm.2019-0009-OA. Epub 2019 Nov 14. PubMed PMID:
31723075; PubMed Central PMCID:
PMC7220826.
Population-scale longitudinal mapping of COVID-19 symptoms, behaviour and testing.
Nat Hum Behav.
2020 Sep;4(9):972-982. doi: 10.1038/s41562-020-00944-2. Epub 2020 Aug 26. PubMed PMID:
32848231; PubMed Central PMCID:
PMC7501153.
Diverse enzymatic activities mediate antiviral immunity in prokaryotes.
Science.
2020 Aug 28;369(6507):1077-1084. doi: 10.1126/science.aba0372. PubMed PMID:
32855333; PubMed Central PMCID:
PMC7985843.
Building an international consortium for tracking coronavirus health status.
Nat Med.
2020 Aug;26(8):1161-1165. doi: 10.1038/s41591-020-0929-x. PubMed PMID:
32488218.
Rapid and accurate species identification for ecological studies and monitoring using CRISPR-based SHERLOCK.
Mol Ecol Resour.
2020 Jul;20(4):961-970. doi: 10.1111/1755-0998.13186. Epub 2020 Jun 13. PubMed PMID:
32396992; PubMed Central PMCID:
PMC7497203.
What would you like to do?