Mucilaginibacter aquatilis sp. nov., Mucilaginibacter arboris sp. nov., and Mucilaginibacter ginkgonis sp. nov., novel bacteria isolated from freshwater and tree bark

Three Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strains, designated as HME9299^T, HMF7410^T and HMF7856^T, were isolated from freshwater and tree bark collected in Yong-in, Republic of Korea. Strains HME9299^T, HMF7410^T and HMF7856^T exhibited the highest 16S rRNA gene sequence similarities of 97.2, 94.4 and 96.4 % to Mucilaginibacter daejeonensis Jip 10^T, Mucilaginibacter terrae CCM 8645^T and Mucilaginibacter phyllosphaerae PP-F2F-G21^T, respectively. Among themselves, the values were 94.1-95.7 %. Phylogenetic analysis of the 16S rRNA gene sequences of the three isolates revealed that they belonged to the genus Mucilaginibacter within the family Sphingobacteriaceae. The predominant fatty acids of three strains were summed feature 3 (comprising C_{16 : 1} ω7c and/or C_{16 : 1} ω6c) and iso-C_{15 : 0}. Strain HME9299^T contained a relatively large amount of C_{16 : 1} ω5c. The predominant respiratory quinone was menaquinone-7. The genome sizes of strains HME9299^T, HMF7410^T and HMF7856^T were 4.33, 4.16 and 3.68 Mbp, respectively, and their DNA G+C contents were 41.6, 38.4 and 43.9 mol%, respectively. Based on the results of the phenotypic, genotypic, chemotaxonomic and phylogenetic investigation, three novel species, Mucilaginibacter aquatilis sp. nov, Mucilaginibacter arboris sp. nov. and Mucilaginibacter ginkgonis sp. nov., are proposed. The type strains are HME9299^T (=KCTC 42122^T=DSM 29146^T), HMF7410^T (=KCTC 62464^T=NBRC 113227^T) and HMF7856^T (=KCTC 72782^T=NBRC 114275^T), respectively.