Abstract
We show that epd (gapB) mutants lacking an erythrose 4-phosphate (E4P) dehydrogenase are impaired for growth on some media and contain less pyridoxal 5'-phosphate (PLP) and pyridoxamine 5'-phosphate (PMP) than their epd+ parent. In contrast to a previous report, we found that gapA epd double mutants lacking the glyceraldehyde 3-phosphate and E4P dehydrogenases are auxotrophic for pyridoxine. These results implicate the GapA and Epd dehydrogenases in de novo PLP and PMP coenzyme biosynthesis.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aldehyde Oxidoreductases / genetics
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Aldehyde Oxidoreductases / metabolism
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins*
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Mutation / genetics
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Oxidoreductases / genetics
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Oxidoreductases / metabolism*
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Pyridoxal Phosphate / biosynthesis
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Pyridoxal Phosphate / metabolism*
Substances
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Escherichia coli Proteins
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Pyridoxal Phosphate
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Oxidoreductases
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Aldehyde Oxidoreductases
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erythrose 4-phosphate dehydrogenase, E coli