RNA-Seq of Kenaf anther:dual-core period - SRA

SRX7581565: RNA-Seq of Kenaf anther:dual-core period
1 ILLUMINA (Illumina NovaSeq 6000) run: 24.6M spots, 7.4G bases, 2.2Gb downloads

Design: Total RNAs from biological replicates 1 of UG93A was extracted from the anther tissue, bead with oligo (dT) were utilized to isolate poly (A) mRNA following the total RNA. A fragmentation buffer was added to break the mRNA into short fragment. Taking these short fragments as templates, double-stranded cDNA was synthesized.Then the synthesized cDNA was subjected to end-repair, phosphorylation and A base addition according to Illuminas library construction protocol.Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerasefor 15 PCR cycles. After quantified by TBS380, the RNAseq libraries were sequenced in single lane on an Illumina Hiseq xten sequencerfor 2150bp paired-end reads.

Submitted by: Guangxi University

Study: Transcriptome de novo assembly and differentially expressed genes related to cytoplasmic male sterility in kenaf (Hibiscus cannabinus L.)

PRJNA602109 • SRP242712 • All experiments • All runs

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To reveal the possible mechanism, a comparative transcriptome analysis of kenaf anthers from a CMS line and its maintainer was conducted using Solexa sequencing.

Sample:

SAMN13881600 • SRS6015295 • All experiments • All runs

Organism: Hibiscus cannabinus

Library:

Name: UG93A_1

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Runs: 1 run, 24.6M spots, 7.4G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR10914153	24,603,115	7.4G	2.2Gb	2021-01-01

ID:: 9900546

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