Single-base extension: Difference between revisions
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The '''Single Base Extension''' (SBE) is a [[biology|biological]] reaction, which can follow after a PCR ([[Polymerase]] Chain Reaction). |
The '''Single Base Extension''' (SBE) is a [[biology|biological]] reaction, which can follow after a PCR ([[Polymerase]] Chain Reaction). |
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The PCR Product is a [[DNA]] sequence which includes a SNP (Single Nucleotide Polymorphism). This DNA-Sequence is used as [[Template]] in a SBE. Another component for this reaction is a special primer, with a biotin - molecule at on side and a special base which is easier destroyable through UV-Light than other bases at the other side. In addition also a special SBE-Polymerase is given to the components of that reaction. That polymerase |
The PCR Product is a [[DNA]] sequence which includes a SNP (Single Nucleotide Polymorphism). This DNA-Sequence is used as [[Template]] in a SBE. Another component for this reaction is a special primer, with a biotin - molecule at on side and a special base which is easier destroyable through UV-Light than other bases at the other side. In addition also a special SBE-Polymerase is given to the components of that reaction. That polymerase synthesizes the primer on the template exact one base before the SNP. After this the polymerase synthezises a ddNTP according to the base pairing on the SNP. When finished its work, the reaction is over. |
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After a SBE follows an SBE-Product-Purification and at the end a MALDI TOF MS to analyse which kind of SNP is in the DNA-Sequence. |
After a SBE follows an SBE-Product-Purification and at the end a MALDI TOF MS to analyse which kind of SNP is in the DNA-Sequence. |
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{{unreferenced|date=November 2006}} |
{{unreferenced|date=November 2006}} |
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Revision as of 21:50, 4 April 2007
The Single Base Extension (SBE) is a biological reaction, which can follow after a PCR (Polymerase Chain Reaction).
The PCR Product is a DNA sequence which includes a SNP (Single Nucleotide Polymorphism). This DNA-Sequence is used as Template in a SBE. Another component for this reaction is a special primer, with a biotin - molecule at on side and a special base which is easier destroyable through UV-Light than other bases at the other side. In addition also a special SBE-Polymerase is given to the components of that reaction. That polymerase synthesizes the primer on the template exact one base before the SNP. After this the polymerase synthezises a ddNTP according to the base pairing on the SNP. When finished its work, the reaction is over. After a SBE follows an SBE-Product-Purification and at the end a MALDI TOF MS to analyse which kind of SNP is in the DNA-Sequence.
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