| abstract |
The present invention discloses the application of mA RNA methylation ncontent, and methylation-related enzymes and binding proteins thereof in the npreparation of a senescence detection kit. The senescence detection kit of the npresent invention determines cell senescence by detecting protein expressions of nm6A RNA methylation content, RNA methylation enzymes METTL3, METTL14, n6 WTAP and KIAA1429, m A RNA demethylation enzymes FTO and ALKBH5, nand m A RNA methylation binding proteins YTHDC1, YTHDF1 and YTHDF2; nthe high expression or low expression of RNA methylation enzyme METTL3 is nused for determining whether the cell senescence is premature or normally nreplicative. The present invention can be applied to the assessment of premature nsenescence, replicative senescence and senescence-related diseases, and thus can nbe widely used in the future.nDrawings nA n22PDL 35PDL 49PDL nPSi PSp nB n120.0 nns n100.0 n80.0 nso4. n60.0 n40.0 n20.0 n'Is n0.0 n22PDL 35PDL 49PDL PSi PSp nFig. 1 n1/3 |