http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-1086199-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0dfa756d8d95385994f1521f13bdf5b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 |
| filingDate | 1979-10-17^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1980-09-23^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c740240c07ebe6e6c45742f2e6986f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f01894fc6641bbc46bf959ebbad3b57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4da6e8c55a051ae08571798abeb03935 |
| publicationDate | 1980-09-23^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-1086199-A |
| titleOfInvention | Kinetic assay for alpha-amylase |
| abstract | ABSTRACT OF THE DISCLOSURE An acid phosphatase assay comprising: a) performing simultaneous reactions at a pH of from about 4.5 to about 6 which comprise; (i) reacting an organic phosphate selected from a group consisting of beta-glycerophosphate, phenyl phosphate. p-nitrophenyl phosphate, alpha-naphthyl phosphate, adenosine-3'-monophosphate, thymolphthnlein monophosphate, and phenol-phthalein monophosphate in the presence of acid phosphatase to release inorganic phosphate; (ii) reacting maltose with phosphate ions in the presence of maltose phosphorylase to form glucose and .beta.-D-glucose-1-phosphate; (iii) reacting .beta.-D-glucose-1-phosphate in the presence of .beta.-D-phosphoglucomutase to form glucose-6-phosphate; and (iv) reacting glucose-6-phosphate in the presence of glucose-6-phosphate dehydrogonase and a co-enzyme selected from a group consisting of .beta.-nicotinamide-adenine dinucleotide, .beta.-nicotinamide-adenine dinucleotide phosphate, and mixtures thereof to form the reduced form of said co-enzyme and 6-phosphogluconate; and (b) measuring the rate of production of said reduced co-enzyme, wherein the acid phosphatase being measured is rate-limiting and wherein a non-phosphate buffer is used to control said pH. |
| priorityDate | 1976-02-13^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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