| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dea97595058f316a875c28a64efb90da |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-81 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-12007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 |
| filingDate |
1998-04-07^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2009-12-08^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_813c398d59a5401a61a0ae6635bd8194
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d611358305181dfbfa67f0716bbc829
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8248ff6728504c54b2faf3986d076156
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b6c39867269e8cd57b692ff65a9f324 |
| publicationDate |
2009-12-08^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2283287-C |
| titleOfInvention |
Enzyme assays |
| abstract |
Enzymes and methods suitable for assaying ATP, and specific applications for such assays are described and claimed. In particular, there is described a recombinant mutant luciferase having a mutation (e.g. t he amino-acid corresponding to amino acid residue number 245 in Photinus pyralis which is such that the K m for ATP of the luciferase is increased e.g. five-fold with respect to that of the corresponding non-mutated enzyme such that it is of the order of 500 .mu.m - 1mM. Also disclosed are luciferases having additional mutations conferring improved thermostability or altered wavelength of emitted light. Recombinant polynucioetides, vectors and host cells are also disclosed, as are methods of assaying the amount of ATP in a material (e.g. cells) optionally in real-time. Also disclosed are test-kits for in vitro assays. |
| priorityDate |
1997-04-11^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |