| abstract |
The present invention provides a tool for the isolation of ck-it expressing cells. This tool consists of a c-kit plasmid targeting vector which is capab le of integrating into the wild type c-kit allele and encodes a chimeric fluorescent protein comprising a nucleolar localisation signal such as, TCOF - 12, RLP313, RPS254 or Fxr2h5.. Said construct generates a condensed, bright, fluorescent signal that can be localised in living tissue and after dissociation. The construct allows visualisation using confocal microscopy a nd allows automated cell sorting of the dissociated cells using amongst others flow cytometry. |