http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102492037-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-14 |
| filingDate | 2011-12-09^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-10-30^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ef8efe201681a514c4b37793a8a1420 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_406f4e39e1c86130fb0e6a23f7019be6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dec5460656c3e4d58ddb9632a14963f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16a8e902c3cc00b6fb1d5feb790ace0e |
| publicationDate | 2013-10-30^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102492037-B |
| titleOfInvention | High affinity fusarium specific single-chain antibody and preparation method thereof |
| abstract | The invention discloses a high affinity fusarium specific single-chain antibody and a preparation method thereof, which includes the following steps: A performing genic mutation to a fusarium graminearum cell wall specific antibody CWP2; B building mutation antibody file bases; C utilizing phage display to select and gather the mutation antibody file bases so as to obtain antibody proteins; D performing PhaseElisa analysis on combining capacity of gathered mutants and fusarium graminearum cell wall proteins; E showing combining strength between the mutants obtained in the ELISA identification step D and a parent antibody CWP2 and the fusarium graminearum cell wall proteins; F showing Westernblot analysis between the high affinity antibody identified by the ELISA and the fusarium graminearum cell wall proteins; and G proving that affinity of the high affinity single-chain antibody and fusarium graminearum cell wall proteins is 15 times of the parent antibody CWP2 when the identified high affinity single-chain antibody and the parent antibody CWP2 perform surface plasma resonance detection. The high affinity single-chain antibody enriches scab resistant sources and establishes the foundation for cultivation of wheat scab high resistant varieties. |
| priorityDate | 2011-12-09^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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