http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103060416-B

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filingDate 2012-12-20^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-05-07^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7a93bee59c9201915868e6afc628437
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publicationDate 2014-05-07^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103060416-B
titleOfInvention Method for cleaning and producing dioscorea zingiberensis saponin with microbial technology adopted
abstract The invention provides a method for cleaning and producing dioscorea zingiberensis saponin with microbial technology adopted, and belongs to the field of bioengineering. The method solves the problems that in the existing dioscorea zingiberensis industry, wastewater and waste residues are large in drainage amount, and dioscorea zingiberensis starch is not utilized effectively. The method includes the following steps: releasing dioscorea zingiberensis saponin with microorganism adopted firstly, obtaining fermentation liquor containing the dioscorea zingiberensis saponin and the starch; and then processing the starch in the fermentation liquor with a two-enzyme method adopted and obtaining hydrolyzed sugar, and further releasing the dioscorea zingiberensis saponin; conducting solid-liquid separation on dioscorea zingiberensis mash; gathering the hydrolyzed sugar and the dioscorea zingiberensis saponin with microfiltration and nanofiltration membrane separation adopted in sequence, or obtaining intracellular products and dioscorea zingiberensis saponin through specific microbial fermentation separation, and obtaining the dioscorea zingiberensis saponin by hydrolysis of glycosidase and a small amount acid and extraction of a small amount of organic solvent on concentrated dioscorea zingiberensis saponin solution. According to the method, the yield coefficient of the dioscorea zingiberensis saponin is high (90-95%), wastewater quantity is little in the process of production, degree of contamination is low, microbial treatment is low in cost and short in use time, the dioscorea zingiberensis starch is recycled fully, and the method has a great industrial application promotion value in the dioscorea zingiberensis industry.
priorityDate 2012-12-20^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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