http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103074309-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_27396494dbf2775fd87cdd0287df497f |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate | 2012-09-11^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-16^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6ea7f31eb2faf8f58abbf72de862baa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7694949b9723dc1e10ff79a8be35050e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf7fe8100e564755203c4a412197cc29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ee23c39a33877ead39ae7dd2c07dbd4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a2484edcc7156130a355436cdca9155 |
| publicationDate | 2014-04-16^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103074309-B |
| titleOfInvention | L-alanine dehydrogenase mutant zymoprotein and preparation method thereof |
| abstract | The present invention discloses L-alanine dehydrogenase mutant zymoprotein and a preparation method thereof. According to a homologous sequence secondary structure comparison result, site-specific mutagenesis of 73th lysine near an L-alanine dehydrogenase activity center of Bacilluspseudofirmus into alanine through PCR is performed to construct a mutant expression vector, and prokaryotic expression and Ni-NTA affinity chromatography purification are performed to obtain a mutant enzyme K73A, wherein specific activity of the obtained mutant enzyme K73A is 202% of specific activity the wild type, and other enzymatic properties are not changed. In addition, a turnover number Kcat on a substrate L-alanine by the mutant zymoprotein is 376.7 min<-1> and is 2.0 times the turnover number Kcat of the wild type, a turnover number Kcat on beta-NAD<+> by the mutant zymoprotein is 290.1 min<-1> and is 2.1 times the turnover number Kcat of the wild type, and the mutant zymoprotein can be applicable for production processes of production of pyruvic acid, L/D-alanine, and the like through improvement biology methods. |
| priorityDate | 2012-09-11^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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