abstract |
The present invention relates to novel microbial 7α-hydroxysteroid dehydrogenase (7α-HSDH) knockout mutants and their use in the production of other HSDHs (e.g. 3α-, 7β- or 12α-HSDH) with different functionalities Use, and the use of the HSDH enzyme produced thereby in the enzymatic reaction of bile acid compounds, and in particular in the production of ursodeoxycholic acid (UDCA). In particular, the present invention relates to novel methods for the synthesis of UDCA. |