http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103305610-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d196f7d35941ecfe5676bc1bb8c37166 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2013-05-30^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-03-18^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d39ead762e39eec4432dbd7cc6e1dd7e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffac93441e8202b1e93e8971d8d62c17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0395adef9a06d4827da5bb5786084463 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea9bed12f458004f7af1d5923d4fb565 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c665b841320957d1ea31ea5ceecd535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bcaca631140fb692f84c20370ab30c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98149d30deb92a34d7d56d76762be169 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2b2efcd85195cabcb302f93615057d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c72e90c32d40d9d3f7fadea3095cc1c0 |
| publicationDate | 2015-03-18^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103305610-B |
| titleOfInvention | Method for screening microsatellite molecular markers of Charybdis feriatus |
| abstract | The invention relates to a method for screening microsatellite molecular markers of Charybdis feriatus. The method comprises the following steps of: extracting and diluting a genomic DNA (Deoxyribose Nucleic Acid) of the Charybdis feriatus for later use; designing a 5' anchor primer and implementing PCR (Polymerase Chain Reaction) amplification; cloning and sequencing amplification products; implementing sequence analysis and microsatellite specific primer design; implementing PCR amplification on genomic DNAs of different individuals of the Charybdis feriatus by the primer; detecting PCR amplification products by denaturing polyacrylamide gel electrophoresis; and determining genotype of each individual according to different migration distances of the amplification products, thus obtaining a polymorphism map of genetic variation of the Charybdis feriatus. The method is simple and safe to operate, high in positive rate, real and reliable in result, and capable of quickly obtaining a polymorphism map of the genetic variation of the Charybdis feriatus. The method is mainly applied to genetic variation analysis and population genetic diversity study of the Charybdis feriatus. |
| priorityDate | 2013-05-30^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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