http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103305611-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d196f7d35941ecfe5676bc1bb8c37166 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-05-30^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-22^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d39ead762e39eec4432dbd7cc6e1dd7e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0395adef9a06d4827da5bb5786084463 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea9bed12f458004f7af1d5923d4fb565 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c72e90c32d40d9d3f7fadea3095cc1c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffac93441e8202b1e93e8971d8d62c17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c665b841320957d1ea31ea5ceecd535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98149d30deb92a34d7d56d76762be169 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bcaca631140fb692f84c20370ab30c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2b2efcd85195cabcb302f93615057d3 |
| publicationDate | 2015-04-22^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103305611-B |
| titleOfInvention | Method for rapidly detecting microsatellite markers of Charybdis feriatus |
| abstract | The invention relates to a method for rapidly detecting microsatellite markers of Charybdis feriatus. The method comprises the following steps of: (1) extracting a genomic DNA (Deoxyribose Nucleic Acid) of the Charybdis feriatus; (2) obtaining a gene sequence containing microsatellite repeats according to the functional gene sequence of the Charybdis feriatus in a GeneBank database; (3) designing a microsatellite marker primer; (4) implementing PCR (Polymerase Chain Reaction) amplification on the genomic DNA of different individuals of the Charybdis feriatus; and (5) detecting denaturing polyacrylamide gel electrophoresis of PCR products. The method has the advantages of rapidness, accuracy, sensitivity and the like, and can intuitively detect the genotype of different individuals of the Charybdis feriatus, so that a polymorphism map of genetic variation of the Charybdis feriatus on a functional gene microsatellite site is obtained quickly; the microsatellite markers can be applied to the fields of analysis on genetic variation and population genetic diversity of the Charybdis feriatus. |
| priorityDate | 2013-05-30^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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