http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103555838-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-125 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2013-10-31^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-09-14^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-09-14^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103555838-B |
titleOfInvention | A kind of miRNA detection probe based on rolling circle amplification reaction, detection method and test kit |
abstract | The invention provides a kind of miRNA detection probe based on rolling circle amplification reaction, detection method and test kit.The probe that the present invention provides includes hair fastener probe and ring-shaped probe, hair fastener probe includes 5 ' side chains (1), ring region (2) and 3 ' side chains (3) successively, 5 ' side chains (1), ring region (2) and 3 ' side chains (3) are single-stranded nucleotide, wherein, 5 ' side chains (1) and ring region (2) have the nucleotide sequence complementary with miRNA to be measured, the partial nucleotide sequence of 5 ' side chains (1) and 3 ' side chains (3) is complementary, and 3 ' side chains (3) have the nucleotide sequence that the partial nucleotide sequence with ring-shaped probe is complementary;The miRNA detection method that the present invention provides, can distinguish miRNA similar to target sequence for miRNA to be measured and target miRNA precursor;And during detection blood sample, need not miRNA extracting and purification. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108845003-A |
priorityDate | 2013-10-31^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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