http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103571850-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2012-07-26^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-09-30^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-09-30^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103571850-B |
titleOfInvention | One grows cotton Phytosulfokine precursor-gene GhPSKP and preparation method and application |
abstract | The invention discloses one to grow cotton Phytosulfokine precursor-gene GhPSKP and preparation method and application, isolate from cotton fiber development different times express spectra one with the EST of other plant PSKP gene very high homology, bioinformatic analysis shows, this EST comprises a complete ORF, by its called after GhPSKP.This gene at elongate fiber high expression in each in period, 10DPA fiber total serum IgE is extracted from Upland Cotton YZ1, utilize ThermoScript II Superscript III by its reverse transcription synthesis cDNA, amplify the full-length cDNA of GhPSKP gene with primer GhPSKPF and GhPSKPR.The PCR primer that amplification obtains is connected into pGEM-T carrier, and screening positive clone also checks order, the full length gene cDNA needed for acquisition.This gene of further confirmation is at cotton fiber rapid elongation stage predominant expression.Use transgenic approach this gene of overexpression in cotton body effectively can promote the elongation of fiber.Utilize the present invention effectively can promote the elongation of cotton fiber, improving cotton fiber quality. |
priorityDate | 2012-07-26^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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