http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103614483-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_30783f5e0439f26fb23d0089d47f0ea6 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2013-12-10^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-06-03^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce8d2f4ede294cc68f82eab9ec28e393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa124d354ed004628e7e80856e30f098 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f48faa9968ca37303aceb1865c52c94 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1fa70a9d3d53cd3a1b1148b7f54584e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ab11d275529710b5f180fd42b3cbae7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7490603f06aef29988c2b5401f7f5ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b01d353c8ad2ab0d7dd2e3459578513d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9f3666d8b40d547bbefa5cbd06fc7fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0478030799dd8544aa956be72a72022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8d12a7da9853919e83142bf21b2d178 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c84039431a2f8241f24a793228745e0 |
| publicationDate | 2015-06-03^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103614483-B |
| titleOfInvention | Method for detecting salmonella invA gene based on rolling circle amplification and gold nanoparticles |
| abstract | The invention discloses a method for detecting salmonella invA gene based on rolling circle amplification and gold nanoparticles. The method comprises the following steps: dropwise adding a capture probe on a clean gold electrode, placing the gold electrode in a refrigerator overnight, taking out and sealing the gold electrode, dropwise adding a sample to be detected on the gold electrode, dropwise adding cyclic DNA after the reaction, reacting, carrying out rolling circle amplification, adding a gold nanoparticle probe, hybridizing and detecting a DPV signal. According to the method disclosed by the invention, the highly conserved invA gene of the salmonella is selected, the probe specifically combined with the invA gene is designed, and the salmonella invA gene is detected by an electrochemical technology in combination with rolling circle amplification and gold nanoparticle technology, so that the sensitivity is greatly perfected, the linear detection range is extended to 100 aM to 10 pM, and the sensitivity is 100 aM. The salmonella detection range in polluted milk is 20 to 6*10<8> CFU ml<-1>, and the lowest detectable limit is 20 CFU ml<-1>. A method for quickly and ultra-sensitively detecting salmonella is created in the invention to greatly improve the detection sensitivity, and the method has the advantages of being miniaturized in detection equipment, convenient, rapid and low in detection cost. |
| priorityDate | 2013-12-10^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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