http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103709242-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-461 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-00 |
| filingDate | 2013-12-25^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-08-26^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-08-26^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103709242-B |
| titleOfInvention | A kind of recombinant protein based on Fugu rubripes (Temmincket Schlegel) FoxO1 gene, preparation method and application |
| abstract | The invention discloses a kind of recombinant protein based on Fugu rubripes (Temmincket Schlegel) FoxO1 gene, preparation method and application, belong to biological technical field.The present invention, according to the cDNA encoding sequence of Fugu rubripes (Temmincket Schlegel) FoxO1 albumen, designs the primer pair for Fugu rubripes (Temmincket Schlegel) FoxO1 albumen coded sequence, carries out RT-PCR amplification after extracting Fugu rubripes (Temmincket Schlegel) liver total RNA with ISOGEN Reagent.RT-PCR product is connected to obtain recombinant plasmid with pGEM-T carrier, and after double digestion, goal gene is connected with expression vector pET-32a (+) again, obtains recombinant expression plasmid and is converted in intestinal bacteria Rosetta (DE3); The solubility expression of recombinant protein is achieved by IPTG induction.Fugu rubripes (Temmincket Schlegel) FoxO1 recombinant protein in the present invention can suppress the expression of peroxisome proliferator activated receptor PPAR γ gene, regulates Fugu rubripes (Temmincket Schlegel) body lipid metabolism balance.The recombinant products of purifying carries out Active MnO2 to fish or feed adds, and can improve the growth of cultured fishes and reduce the lipid content of fish, making delicious meat good to eat. |
| priorityDate | 2013-12-25^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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