http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103911368-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2014-03-30^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-08-17^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-08-17^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103911368-B |
titleOfInvention | Based on immuno-precipitation from Oryza sativa L., extract polysomal method |
abstract | The invention belongs to technical field of molecular biology, a kind of based on immuno-precipitation from Oryza sativa L., extract polysomal method.The present invention is based on immunization, use the ribosome mRNA complex of the method enrichment Flag label labelling of affinity chromatograph, select other metal ion in EGTA chelating tissue homogenate, and add chloromycetin and cycloheximide suppression ribosome and the dissociating of mRNA, wherein improve extracted solution and eluting solution formula, material homogenate and condensing mode, use the EDTA eluting ribosome mRNA complex of final concentration of 0.1M.Present invention expection, by separating 24 important New function genes participating in low temperature stress, is normally carried out for the plant growing under low temperature stress and multiplies providing safeguard, finally play a significant role on yield is built up. |
priorityDate | 2014-03-30^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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