http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104830748-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2015-06-02^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-04-06^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-04-06^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104830748-B |
| titleOfInvention | A kind of reduction hemB gene expressions improve the method that Escherichia coli synthesize 5 amino-laevulic acids |
| abstract | The invention discloses a kind of method that reduction hemB gene expressions improve Escherichia coli 5 amino-laevulic acids of synthesis, belong to metabolic engineering and field of microbial fermentation.The present invention is using the overexpression glutamy tRNA reductases of carrier pRSFDuet 1, on the basis of paddy ammonium aldehyde aminopherase and coproporphyrinogen III oxidizing ferment and the overexpression uroporphyrinogen III synthase of use carrier pETDuet 1, promoter by transforming hemB genes in genomic level investigates its influence to ALA accumulation to regulate and control its expression.Verified by shake flask fermentation, purpose product ALA yield is significantly improved, and ALA yield is 2680mg/L during 30h. |
| priorityDate | 2015-06-02^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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