http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105176879-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2015-10-14^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-12-12^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-12-12^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105176879-B |
| titleOfInvention | A kind of method that knockout argCJBD improves recombined bacillus subtilis acetylglucosamine yield |
| abstract | The invention discloses a kind of method that knockout argCJBD improves recombined bacillus subtilis acetylglucosamine yield, belong to field of genetic engineering.The present invention is with recombined bacillus subtilis BSGN6 P xylA ‑glmS‑P 43 GNA1 is as starting strain, acetylglutamate semialdehyde dehydrogenase encoding gene (argC), ornithine acetyltransferase encoding gene (argJ), acetylglutamate kinase encoding gene (argB) and acetyl-ornithine transaminase encoding gene (argD) are knocked out by homologous recombination, Host Strains have been blocked to generate arginic approach by glutamic acid, glutamic acid is promoted to be converted into the reaction of glutamine, and glutamine is the amino group donor of acetylglucosamine synthesis, so as to improve the yield of acetylglucosamine.During the shake flask fermentation using semisynthetic medium, the yield for knocking out argCJBD recombined bacillus subtilis acetylglucosamine reaches 6.4g/L, than improving 16.3% before knockout.Glucosamine is produced for further metabolic engineering bacillus subtilis to lay a good foundation. |
| priorityDate | 2015-10-14^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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