http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105602879-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y503-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-61 |
| filingDate | 2016-01-26^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-09-03^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-09-03^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105602879-B |
| titleOfInvention | Engineering strain, construction method and its application of one plant of efficient secretion D-Psicose 3- epimerase |
| abstract | The invention belongs to technical field of bioengineering, and in particular to the engineering strain and its construction method of one plant of efficient secretion D-Psicose 3- epimerase.The present invention is obtained first from cud bacterium Ruminococcus The D-Psicose 3- epimerism enzyme gene of sp.5_1_39B_FAA rdpe , construct recombinant expression plasmid pMA5-RDPE and convert bacillus subtilis, realize RDPE constitutive and secretive expression in bacillus subtilis.By comparing three sugared inducible promoters, optimal inducible promoter P is obtained xylA , and significantly improve the secretion level of RDPE.By knocking out xylose utilization genes xylAB ( xylA With xylB ), the xylose metabolism approach of bacillus subtilis has been blocked, the secretory volume of RDPE is further improved, and the optimal induced concentration of inducer xylose is made to be reduced to 0.5% by 4.0%.Finally, using batch feeding mode, engineered strain 1A751SD-XR is evaluated in 7.5 L fermentors, the secretion level highest of RDPE can achieve 95 U/mL and 2.6 g/L. |
| priorityDate | 2016-01-26^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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