http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105647950-B
Outgoing Links
Predicate | Object |
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classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-38 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2014-11-10^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-07-12^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-07-12^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105647950-B |
titleOfInvention | It is a kind of improve vitamin B12 yield recombinant bacterium construction method and its application |
abstract | The invention discloses a kind of construction method for improving vitamin B12 yield recombinant bacterium and its applications, method provided by the invention, for 1) or 2): 1) method shown in, include the following steps: to import combination any one or more in the encoding gene of the encoding gene of at least following 18 enzymes or following 18 enzymes in purpose bacterium A jointly, obtains recombinant bacterium A;2) method shown in includes the following steps: to import combination any one or more in the encoding gene of the encoding gene of at least following 13 enzymes or following 13 enzymes in purpose bacterium B jointly, obtains recombinant bacterium B.In channel genes host strain required for the present invention synthesizes 13 or 18 kind of vitamin B12, various different degrees of raisings are obtained by the yield that shake flat experiment can detecte vitamin B12 in various recombinant bacteriums, the volume range of various recombinant bacterium vitamin B12s is 44mg/L-400mg/L. |
priorityDate | 2014-11-10^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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