http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106399299-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-9126 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 |
| filingDate | 2016-09-29^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-29^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-29^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106399299-B |
| titleOfInvention | A kind of method and application improving large fragment Geobacillus stearothermophilus DNA polymerase activity by point mutation |
| abstract | The present invention discloses a kind of method and application that large fragment Geobacillus stearothermophilus DNA polymerase activity is improved by point mutation, belongs to the DNA recombinant expression technical field of genetic engineering field.The gene source of enzyme is in Geobacillus stearothermophilus (Geobacillus stearothermophilus).Large fragment Bst archaeal dna polymerase plasmid is obtained by genetic recombination.310th amino acids G of large fragment Bst archaeal dna polymerase is sported into L or A, or 510 amino acids D are sported into E, mutational site amino acid is conserved amino acid.As the result is shown compared with wild type Bst archaeal dna polymerase, the polymerization efficiency of mutant G310L, G310A and D540E are significantly increased, and are all higher than commercialization Bst archaeal dna polymerase, have biggish application value, provide convenience for the production domesticization of Bst archaeal dna polymerase. |
| priorityDate | 2016-09-29^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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