http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106908402-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-3185 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-2866 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-314 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2017-03-17^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-03-24^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-03-24^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-106908402-B |
| titleOfInvention | Sample preparation method for proteomics analysis of broad bean root system |
| abstract | The application discloses a sample preparation method for proteomics analysis of broad bean root systems, which comprises the following steps: taking fresh broad bean root systems; grinding by liquid nitrogen; extracting with phenol; tris equilibrium phenol saturated solution; centrifuging and collecting the upper phenol layer; 0.1mol/L ammonium acetate-methanol solution; centrifuging and collecting the precipitate; washing with methanol; centrifuging and collecting the precipitate; drying the precipitate in vacuum; adding a sample lysis solution; centrifuging and taking a supernatant; protein quantification; subpackaging and freezing; hydrating the sample; the sample preparation was complete. The invention takes the root system of the broad bean as an experimental material, and fills the blank of the two-dimensional electrophoresis technology of the root system of the broad bean. The method has the advantages of simple experimental steps, easy operation, good repeatability and easy learning and mastering of technicians with experimental backgrounds. The experimental reagents selected in the invention are conventional reagents for dimensional electrophoresis, no expensive and rare reagents are used, and the experimental operation cost is low. |
| priorityDate | 2017-03-17^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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