| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_653c418e41edbb4e1ce8bc5e53e70cec |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-922
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91011 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate |
2018-07-11^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c45eeea0b1e96bc165bc30ca76eb645
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a616f0763ff34c4808bd0cffcce02a42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fe524e959a8a69c87a4d03abcbb6d19 |
| publicationDate |
2018-12-18^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-109022544-A |
| titleOfInvention |
A kit and method for detecting DNA methyltransferase activity |
| abstract |
The invention discloses a method for detecting DNA methyltransferase activity based on single ribonucleotide repair-mediated ligation-dependent circular signal amplification, (1) adding a hairpin substrate acting on Dam MTase to the excision reaction buffer solution The cleavage reaction will produce a 24-nt product; (2) Add the above cleavage product to the amplification reaction buffer to perform a cyclic ligation-dependent strand displacement amplification reaction, which will generate an enhanced fluorescent signal in a cycle. By detecting the fluorescent signal The strength of the Dam MTase can be used to determine the activity of Dam MTase; the invention can effectively inhibit the non-specific amplification independent of the target and template/primer, thereby greatly reducing the background signal, and finally realizing the exponential amplification of the signal. The method has high sensitivity and specificity The detection limit can reach 4.8×10 ‑6 U/mL. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110643677-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112094889-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113462755-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111944873-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111944873-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111154839-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111154839-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110643677-A |
| priorityDate |
2018-07-11^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |