http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109254101-B

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filingDate 2017-07-13^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-07-09^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-07-09^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-109254101-B
titleOfInvention Method for purifying and analyzing breast milk acid oligosaccharide
abstract The invention relates to a method for purifying and analyzing breast milk acid oligosaccharide. The method is characterized in that: a sample is subjected to low-temperature centrifugal degreasing, an organic solvent is added for precipitation and protein removal, then the interference of lactose and neutral oligosaccharide is removed through an online purification column, the obtained acidic sugar is separated through a hydrophilic interaction chromatographic column, and the structure, the composition and the content information of the breast milk acidic sugar are obtained through evaporative light, electrospray, ultraviolet or mass spectrometry detection. The method has the characteristics of good selectivity, good stability, simple and controllable operation, high flux and the like, and is suitable for rapid and high-flux analysis of mammal milk and human milk samples.
priorityDate 2017-07-13^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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