| abstract |
The present invention discloses a monoclonal antibody comprising a light chain and a heavy chain, the amino acid sequence of the light chain is shown in SEQ ID NO: 1, and the amino acid sequence of the heavy chain is shown in SEQ ID NO: 2; the present invention will identify Zika The polyclonal antibody R1 of viral NS1 protein and the mouse monoclonal antibody 1F12 were used in the preparation of Zika virus NS1 protein double-antibody sandwich ELISA detection reagent, and a rapid detection method of Zika virus NS1 protein double-antibody sandwich ELISA was established, which can detect quickly and accurately Zika virus; this technology integrates the specificity of antibodies and the high sensitivity of ELISA. It is accurate, fast, efficient, specific, sensitive, and does not require virus isolation and RNA extraction. It is suitable for import and export quarantine and patient infection. On-site rapid detection of Zika virus is of great significance for controlling the epidemic, ensuring national security, and guiding the use of clinical drugs in a timely and accurate manner. |