http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109507429-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b4711b7defc1422cdb7f86d2c95657bf |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2018-02-14^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d646f88fee0448ce62c2787ea5dc0a65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2e9b09b92a47181bf6fcd32edea6e69 |
| publicationDate | 2019-03-22^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109507429-A |
| titleOfInvention | A method for the identification and analysis of site-specific interacting proteins based on CRISPR/cas9 and peroxidase APEX2 systems |
| abstract | The invention belongs to the field of biotechnology, and relates to a method for identifying and analyzing specific site-interacting proteins based on CRISPR/cas9 and peroxidase APEX2 systems, including steps, transformation of plasmids; Biotin labeling; detection and analysis of biotin-labeled protein complexes and enriched proteins using streptomycin magnetic beads. The experimental analysis results show that this method is suitable for the research and analysis of proteins at any given chromosomal location; at the same time, the idea of combining the CRISPR gene editing system with APEX2 for site-specific protein analysis is also applicable to other genome editing methods ( such as TALEN) binding to APEX2. The method is simple in operation, high in sensitivity, high in specificity, and good in reproducibility, and is suitable for studying chromatin local interacting proteins at any given chromosomal location. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110702916-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110702916-B |
| priorityDate | 2018-02-14^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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