http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109652459-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-706 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-075 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43572 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 |
| filingDate | 2018-12-29^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-08-07^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-08-07^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109652459-B |
| titleOfInvention | Bee gene editing method based on CRISPR/Cas9 |
| abstract | The invention discloses a bee gene editing method and a gene editing material based on CRISPR/Cas9, which comprises the following steps: s1, determining the sgRNA sequence of the target gene; s2, synthesizing sgRNA of the target gene; s3, microinjecting the editing material on the dorsal side of the tail part or the ventral side close to the head end of the bee egg, and performing the late incubation of the injected egg; s4, PCR amplification of the target gene of the injected ovum; and S5, sequencing the PCR amplification product obtained in the step S4 to obtain a gene editing result of the target gene. The injection site in bee gene editing is changed from the tail part to the ventral side close to the head end; meanwhile, the sgRNA + Cas9 protein mixture is used as a gene editing material in the bee for the first time, the G0 generation double-knock Indel mutant is efficiently obtained, the breeding process of breeding the double-knock mutant worker bee is greatly simplified, and the ecological risk of bee gene editing research is reduced. |
| priorityDate | 2018-12-29^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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