http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110283881-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2411 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01018 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26 |
| filingDate | 2019-06-24^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-06^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-06^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110283881-B |
| titleOfInvention | Method for improving enzyme stability and application thereof |
| abstract | The invention discloses a method for improving enzyme stability and application thereof, belonging to the technical field of protein engineering. The screening method can more accurately obtain the amino acid residues in the enzyme related to the enzyme stability, particularly the enzyme thermal stability; according to the invention, the selected enzyme has the advantages that the alternative amino acid residues related to the stability of the enzyme are mutated into cysteine, and a disulfide bond is formed between the cysteine and the adjacent endogenous reference amino acid residues to construct the enzyme with higher thermal stability, so that the half-life period of the enzyme is prolonged by 1.5-2.0 times after the enzyme is subjected to heat preservation at 65 ℃; the invention has very important significance for improving the industrial application value of the enzyme, and has the advantages of simple operation, safety, no toxicity, good heritability and good application prospect. |
| priorityDate | 2019-06-24^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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