http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110791468-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P33-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0004 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-32 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P33-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2019-10-14^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-23^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-23^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110791468-B |
| titleOfInvention | Construction method and application of mycobacterium genetic engineering bacteria |
| abstract | The invention discloses a construction method and application of a mycobacterium genetic engineering bacterium, belonging to the technical field of genetic engineering. After the recombinant plasmid is transformed into a mycobacterium LY-1 cell, sgRNA can recognize a specific region on a genome and guide Cas9 protein to be combined to a target site, and under the action of the protein, a gap of double-strand break is formed at the target site, and most cells die: the homologous repair sequence introduced with the recombinant plasmid is integrated to the gap through double exchange under the action of recombinase, so that cells successfully repaired by gene homology survive, and artificially designed genotypes such as gene inactivation, foreign gene insertion and the like are formed. The CRISPR-Cas9 system constructed by the invention has the knockout efficiency of 60% aiming at the same gene, and is more convenient and efficient compared with the existing mycobacteria gene editing mode. |
| priorityDate | 2019-10-14^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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