| abstract |
The invention provides a kit and a detection method for detecting PD-L1 gene mutation in peripheral blood circulating tumor cells of patients with small cell lung cancer, belonging to the technical field of molecular biology. Including Diluent, Destaining Solution, Staining Solution A, Staining Solution B, PD‑L1 (Human) Primary Antibody, Goat Anti-Human IgG/HRP, 0.1% Triton X‑100, 0.3% H 2 O 2 , Reagent A, Reagent B , 6×PBS buffer; the detection method provided by the present invention can detect PD-L1 expression in patients with advanced or recurrent small cell lung cancer without obtaining tissue specimens by needle biopsy. The technique is minimally invasive and enables real-time detection. It can avoid false positive results caused by edge effects that may occur in the staining process, has good stability, reduces cell loss, and improves detection accuracy. |