http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112625985-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-03012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y206-01021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-01002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01015 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P39-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-0104 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01031 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P39-00 |
| filingDate | 2020-12-04^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-31^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-31^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112625985-B |
| titleOfInvention | Genetically engineered bacterium for high yield of beta-alanine and co-culture preparation of D-pantothenic acid |
| abstract | The invention relates to a gene engineering bacterium for high yield of beta-alanine, a co-culture preparation method of D-pantothenic acid, a construction method of the co-culture preparation method of the gene engineering bacterium, and application of the co-culture preparation method of the gene engineering bacterium to preparation of the D-pantothenic acid. The invention uses the Trc promoter and RBS sequence from pTrc99A to replace the original promoters of panD, aspC and ppc genes on the genome to strengthen the synthesis of beta-alanine, and knocks out the genes pykA and pykF to block the consumption of PEP and transform the glucose uptake path of escherichia coli, strengthen the non-PTS transport system to block the PTS transport system, and accumulate the synthesized precursor PEP; on the basis, heterologous aspartate decarboxylase genes panD and aspC of E.coli W3110 are introduced to strengthen the enzyme activity of key enzymes, enhance the supply and transformation of beta-alanine precursors, and gdhA genes of E.coli W3110 are introduced to strengthen the cyclic regeneration of coenzyme NADP/DNAPH, so that the titer of the beta-alanine is finally improved from 0 to 2.48 g/L. The strain and the previous D-pantothenic acid producing strain DPA21/pBCST3 are subjected to preliminary co-culture system construction, the inoculation ratio is optimized, and when the inoculation ratio of the two strains is 1:1, the co-culture strain can produce 3.08g/L of D-pantothenic acid in the same fermentation medium. |
| priorityDate | 2020-12-04^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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